Study of laser-induced autofluorescence emission spectra from normal and malignant bronchial epithelium

被引：0

作者：

Bulgakova, Natalia ^{[1
]}

Sokolov, Victor ^{[2
]}

Telegina, Larisa ^{[2
]}

Vereshchagin, Konstantin ^{[1
]}

Frank, Georgy ^{[2
]}

Chissov, Valery ^{[2
]}

机构：

[1] A.M. Prokhorov General Physics Institute of the Russian Academy of Sciences, 119991 Moscow

[2] P. A. Hertzen Moscow Research Oncological Institute, 125284 Moscow

来源：

Photonics and Lasers in Medicine | 2013年 / 2卷 / 02期

关键词：

Laser-induced autofluorescence; Lung cancer; Optical diagnosis; Spectroscopy;

D O I：

10.1515/plm-2013-0002

中图分类号：

学科分类号：

摘要：

Objective: Autofluorescence bronchoscopy (AFB) is currently considered the most promising diagnosis modality for the detection of early stage lung cancer with a sensitivity which normally exceeds 90%, although its specificity is rather low. The number of false positive results can be reduced by the combination of autofluorescence (AF) imaging with quantitative spectroscopy. The aim of this study was to reveal additional spectral characteristics of AF emission which may have the potential to maximize the endogenous contrast between normal mucosa and bronchial malignancy in vivo. Material and method: AF emission spectra were recorded at 532 nm laser excitation in the course of AFB of totally 20 patients with central-type X-ray negative lung cancer. According to the results of histopathological analysis, two sets were made up of the spectral data corresponding to normal bronchial epithelium and malignant lesions. Results: Comparative studies of nine basic spectral characteristics of in vivo AF spectra recorded from normal and malignant bronchial epithelium were performed. Statistical analysis and probabilistic estimations revealed that endogenous contrast between normal bronchial epithelium and early stage lung cancer in the 550 - 800 nm spectral range includes at least five spectral characteristics. The following characteristics were found to be considered as diagnostically informative at 532 nm excitation: i) the maximum spectral intensity, ii) the integral intensity within the range of 550 - 800 nm, iii) the position of the maximum spectral intensity, iv) the center of gravity and v) the relative shape parameter. Conclusion: Real time measurements and estimation of all important spectral information on the basis of the probabilistic approach is assumed to be useful for real time recommendations for biopsies and for minimizing the number of false positive results during the course of AFB. For the realization of this approach, further trials are necessary to collect a large number of AF spectra samples, corresponding to different tissue pathologies such as inflammation, metaplasia, and dysplasia.

引用

页码：93 / 99

页数：6

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