Development of new amperometric biosensors based on organic-inorganic hybrid ultra-thin films

The hybrid ultra-thin films composed of cationic long-alkyl chain molecules and nano-size Prussian Blue clusters were obtained by the Langmuir-Blodgett (LB) technique. It was found that the LB films are quite useful as the starting material for building the high performance biosensors based on oxidative enzymes. The biosensors were easily prepared by a simple immersion of the LB films into enzyme solution, then they work under a very low potential range avoiding the interferent current. We demonstrated that the process is applicable to prepare many types of biosensors just by changing the immobilized enzymes. © 2010 The Institute of Electrical Engineers of Japan.