Flubendiamide provokes oxidative stress, inflammation, miRNAs alteration, and cell cycle deregulation in human prostate epithelial cells: The attenuation impact of synthesized nano-selenium using Trichoderma aureoviride

被引：1

作者：

Department of Pesticides, Faculty of Agriculture, Menoufia University, Egypt ^{[1
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机构：

[1] Department of Pesticides, Faculty of Agriculture, Menoufia University

[2] Department of Pharmacology, Faculty of Medicine, Al-Azhar University

[3] Department of Genetics, Faculty of Agriculture, Menoufia University

[4] Department of Clinical Biochemistry and Molecular Diagnostics, National Liver Institute, Menoufia University

[5] Department of Medical Microbiology and Immunology, Faculty of Medicine, Menoufia University

[6] Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Tanta University

[7] Department of Clinical Medical Sciences, College of Medicine, Dar Al-Uloom University, Riyadh

[8] Department of Clinical Pathology, Faculty of Medicine, Menoufia University

[9] Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Menoufia University

[10] Department of Biological Sciences, College of Sciences and Art, King Abdulaziz University, Rabigh

[11] Department of Community and Public Health, Kasr El Aini Faculty of Medicine, Cairo University

[12] Department of Community Medicine, Ras Al Khaimah Medical and Health Science University

[13] Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Benha University

来源：

Chemosphere |

关键词：

Flubendiamide; Nano-selenium particles; Prostate cells; Trichoderma aureoviride;

D O I：

10.1016/j.chemosphere.2024.143305

中图分类号：

学科分类号：

摘要：

Flubendiamide (FBD) is a novel diamide insecticide extensively used with potential human health hazards. This research aimed to examine the effects of FBD on PrEC prostate epithelial cells, including Oxidative stress, pro-inflammatory responses, modifications in the expression of oncogenic and suppressor miRNAs and their target proteins, disruption of the cell cycle, and apoptosis. Additionally, the research investigated the potential alleviative effect of T-SeNPs, which are selenium nanoparticles biosynthesized by Trichoderma aureoviride, against the toxicity induced by FBD. Selenium nanoparticles were herein synthesized by Trichoderma aureoviride. The major capping metabolites in synthesized T-SeNPs were Isochiapin B and Quercetin 7,3′,4′-trimethyl ether. T-SeNPs showed a spherical shape and an average size between 57 and 96.6 nm. FBD exposure (12 μM) for 14 days induced oxidative stress and inflammatory responses via overexpression of NF-κB family members. It also distinctly caused upregulation of miR-221, miR-222, and E2F2, escorted by downregulation of miR-17, miR-20a, and P27kip1. FBD encouraged PrEC cells to halt at the G1/S checkpoint. Apoptotic cells were drastically increased in FBD-treated sets. Treatment of T-SeNPs simultaneously with FBD revealed its antioxidant, anti-inflammatory, and antitumor activities in counteracting FBD-induced toxicity. Our findings shed light on the potential FBD toxicity that may account for the neoplastic transformation of epithelial cells in the prostate and the mitigating activity of eco-friendly synthesized T-SeNPs. © 2024 Elsevier Ltd

引用

共 159 条

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