Physicochemical Properties of Soy Lipophilic Protein-Hydroxypropyl Methylcellulose Complex under Ultra-high Pressure

被引:0
|
作者
Li Y. [1 ]
Zhong M. [1 ]
Liao Y. [1 ]
Xie F. [1 ]
Sun Y. [1 ]
Qi B. [1 ]
机构
[1] College of Food Science, Northeast Agricultural University, Harbin
关键词
Complex; Hydroxypropyl methyl cellulose; Soy lipophilic protein; Ultra-high pressure;
D O I
10.6041/j.issn.1000-1298.2019.12.042
中图分类号
学科分类号
摘要
Soy lipophilic protein (LP) and hydroxypropyl methylcellulose (HPMC) can form an LP-HPMC complex spontaneously after being combined under neutral conditions (pH value 7.4), but there are still some self-assembled LP and HPMC presented in solution. At present, the research still can not achieve the maximum compounding degree of LP-HPMC and the target of the most binding sites. Fluorescence and infrared spectroscopy were used to study the effects of different ultra-high pressure treatments on the degree of LP-HPMC compounding and the force. The effects of ultra-high pressure on structure-activity relationship of structural changes and functional properties of LP-HPMC were analyzed by emulsion activity and emulsion stability measurement, surface hydrophobicity analysis and dynamic light scattering particle size analysis. The results showed that the LP-HPMC complex was bound by non-covalent interaction and the secondary structure of the protein was changed accordingly. When the pressure was 400 MPa, the maximum solubility and minimum surface hydrophobic value of the composite were 41.1% and 57 832, respectively, and the emulsification activity and emulsion stability index were also the best. © 2019, Chinese Society of Agricultural Machinery. All right reserved.
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页码:365 / 371
页数:6
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共 30 条
  • [1] Gao Z.M., Wang J.M., Wu N.N., Et al., Formation of complex interface and stability of oil-in-water (O/W) emulsion prepared by soy lipophilic protein nanoparticles, Journal of Agricultural & Food Chemistry, 61, 32, pp. 7838-7847, (2013)
  • [2] Kato A., Osako Y., Matsudomi N., Et al., Changes in the emulsifying and foaming properties of proteins during heat denaturation, Agricultural and Biological Chemistry, 47, 1, pp. 33-37, (1983)
  • [3] Knorr D., Heinz V., Buckow R., High pressure application for food biopolymers, BBA-Proteins and Proteomics, 1764, 3, pp. 619-631, (2006)
  • [4] Floury J., Desrumaux A., Legrand J., Effect of ultra-high-pressure homogenization on structure and on rheological properties of soy protein-stabilized emulsions, Journal of Food Science, 67, 9, pp. 3388-3395, (2006)
  • [5] Li B., Study on the changes of colloidal properties of protein and polysaccharide in ultrahigh pressure treatment and its mechanism, (1997)
  • [6] Dickinson E., Hydrocolloids as emulsifiers and emulsion stabilizers, Food Hydrocolloids, 23, 6, pp. 1473-1482, (2009)
  • [7] Oscar E.P., Cecilio C.S., Juan M.R.P., Et al., Adsorption dynamics and surface activity at equilibrium of whey proteins and hydroxypropyl-methyl-cellulose mixtures at the air-water interface, Food Hydrocolloids, 21, 5, pp. 794-803, (2007)
  • [8] Liu L., Intercellular interaction of sodium caseinate-polysaccharide and its effect on emulsion stability, (2011)
  • [9] Zhao M., Long Z., Zhao Q., Et al., Effects of protein dosage and ratio on physical and chemical properties of whipped cream, Journal of Jilin University (Engineering Science), 44, 5, pp. 1-6, (2014)
  • [10] Xu H., Zheng H., Zhu X., Et al., Analysis of modification and application prospect of soy protein concentrate, Food Science, 33, 9, pp. 331-334, (2012)