Preparation and application of polydopamine/sulfobutyl ether-β-cyclodextrin coated open tube capillary electrochromatographic column

被引：0

作者：

Huang, Ziwei ^{[1
]}

Huo, Hongyi ^{[1
]}

Long, Ke ^{[1
]}

Shi, Shuang ^{[1
]}

Guan, Jin ^{[1
]}

机构：

[1] College of Science, Shenyang University of Chemical Technology, Shenyang,110142, China

来源：

Chinese Journal of Analysis Laboratory | 2024年 / 43卷 / 04期

关键词：

Enantiomers;

D O I：

10.13595/j.cnki.issn1000-0720.2022.120102

中图分类号：

学科分类号：

摘要：

An open tube capillary electrochromatographic column was prepared using polydopamine/sulfobutyl ether-β-Cyclodextrin (PDA/SBE-β-CD） as chiral stationary phase. The optimal conditions of fabricated capillary column were as follows： SBE-β-CD concentration of 10.0 mg/mL, dopamine concentration of 5.0 mg/mL, and coating time of 2 h. The fabricated capillary column was characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. Furthermore, the chiral separation effectiveness of the fabricated capillary column was evaluated by capillary electrochromatography (CEC） using enantiomers of omeprazole as model analyte. The enantiomers of omeprazole were resolved within 13 min with high resolution of 5.82 using 110 mmol/L Tris-H3PO4 buffer (pH 6.0） at an applied voltage of 15 kV. Good linearities were obtained in the concentration range of 50-600 µg/mL with the correlation coefficients of r 2 =0.9958 for the enantiomers of omeprazole. And for the migrated time and peak areas, the relative standard deviations (RSDs）were below 2.8% and 4.8%, respectively. The results reveal that PDA/SBE-β-CD has great potential as chiral stationary phases for enantio separation in CEC. © 2024, Youke Publishing Co.,Ltd. All rights reserved.

引用

页码：497 / 501