Preliminary Results of the Effects of Localized High-Dose Radiotherapy Combined with Total Body Low-Dose Irradiation on Tumor Growth and Stimulating the Immune System in Tumor-Bearing Mice

被引：0
作者：
Toosi M.T.B. [1 ]
Torabinejad S. [3 ]
Soleymanifard S. [1 ]
Sankian M. [4 ]
Aledavood S.A. [5 ]
Shamili F.H. [4 ]
Lavi F. [4 ]
Kasiri A. [2 ]
机构：
[1] Medical had, Iran Physics Re-search Center, Mashhad University of Medical Sciences, Mashhad
[2] Department of Medical Physics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad
[3] Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran
[4] Immunology Research Center, Mashhad University of Medical Sciences, Mashhad
[5] Cancer Research Center, Mashhad University of Medical Sciences, Mashhad
来源：
Journal of Biomedical Physics and Engineering | 2023年 / 13卷 / 04期
关键词：
IFN-γ; Immune System; Radiotherapy; Whole-Body Irradiation;
D O I：
10.31661/jbpe.v0i0.2009-1179
中图分类号：
学科分类号：
摘要：
Background: The immune system plays an extensive role in eliminating tumor cells. On the other hand, low-dose irradiation stimulates the immune system. Objective: The present study aimed to investigate the therapeutic outcomes of localized high-dose radiotherapy (LH) alone and combined with total body low-dose irradiation (TB). Material and Methods: In this experimental study, B16F0 tumor cells were injected into the right flank of C57JL/6 mice. The mice were treated with LH alone (13 Gy X-rays to the tumor surface) (LH group) or combined with TB (85 mGy X-rays at the skin) (TB+LH group). Then the tumor volume, the mice’s lifespan, the number of lymphocytes extracted from the spleen, and interferon gamma (IFN-γ) production were measured. Results: Reduced number of lymphocytes, compared to non-irradiated mice (con-trol group), was observed in LH and TB+LH groups. However, the identical number of cultured lymphocytes produced a higher level of IFN-γ in irradiated groups. Comparing the irradiated groups, the number of lymphocytes and their IFN-γ production, tumor growth control, and the mice’s lifespan were statistically higher in TB+LH group. Conclusion: Observing a higher level of IFN-γ in TB+LH group compared to LH group indicates that low-dose radiation enhanced the stimulating effects of high-dose radiation on the immune system. It caused the mice in TB+LH group to have a more prolonged lifespan and a lower tumor growth rate. Therefore, it is worth our attention for future studies to investigate whether total body low-dose irradiation can be utilized before radiotherapy to enhance its efficiency. © 2023, Shiraz University of Medical Sciences. All rights reserved.
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页码：323 / 332
页数：9
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