Isobaric terminal labeling tandem mass spectrometric quantification: A high throughput and accurate biomarker screening technique

被引：0
|
作者：
Wang L. [1 ]
Xie L. [1 ]
Lu H. [1 ]
机构：
[1] Department of Chemistry, Institutes of Biomedical Sciences, Fudan University, Shanghai
来源：
Kexue Tongbao/Chinese Science Bulletin | 2016年 / 61卷 / 4-5期
关键词：
Biomarker screening; Isobaric terminal labeling; Mass spectrometry; Quantitative proteomics;
D O I：
10.1360/N972015-00990
中图分类号：
学科分类号：
摘要：
More specific and sensitive biomarkers in early diagnosis and prognosis are required for curing and preventing cancer. Quantitative proteomics methods are widely used in biomarker discovery because of high accuracy, sensitivity and specificity. In the isobaric terminal labeling quantification, peptide terminis are separately derivatized with complementary isotopically labeled reagents. As a result, the peptide gain the same molecular weight in the MS spectrum, and all N- and C-terminal fragment ions will provide quantification data for each peptide throughout the MS/MS spectrum. Because of the simple operation steps, high accuracy and high throughput, such method offers a wide range of ideas for biomarker discovery and screening. We reviewed here all current developed isobaric terminal labeling quantification techniques and their applications in cancer biomarker research. © 2016, Science Press. All right reserved.
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页码：432 / 441
页数：9
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