Optimization the Electric-Filter Paper Spray Ionization and Application on Rapid Analysis

被引：0
作者：
Jin L.-Y. [1 ]
Wang W.-M. [1 ]
Xie J.-H. [1 ]
Xu F.-X. [1 ]
Ding C.-F. [1 ]
机构：
[1] Institute of Mass Spectrometry Technology and Application, Zhejiang Provincial Key Laboratory of Advanced Mass Spectrometry Technology and Molecular Detection, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo
来源：
Journal of Chinese Mass Spectrometry Society | 2021年 / 42卷 / 04期
关键词：
Ambient ionization; Complex matrix analysis; Ion trap mass spectrometry;
D O I：
10.7538/zpxb.2021.0040
中图分类号：
学科分类号：
摘要：
Traditional ion sources generally require complex sample pre-processing for the analytes, which is time-consuming and labor-intensive, and cannot meet the requirements of rapid on-site detection, this problem greatly restricts the scope of application of the mass spectrometer. Atmospheric pressure ion source is a new type of ionization technology which has emerged in the last ten years, and it is also one of the hotspots in the application of mass spectrometry technology in life sciences, environmental protection, medicine and health, food safety and other fields. With the wider application of mass spectrometry technology, direct and rapid analysis of the composition and content of complex system has become more and more important. Based on the developed miniaturized ion trap mass spectrometer and electric-filter paper spray ion source, this paper conducted in-depth experimental research on their analytical performance and application. With the aim of improving the signal intensity and detection sensitivity of the mass spectra, the aperture of the filter paper, the angle between the tip of the ion source and the horizontal plane, the distance between the ion source and the mass spectrometry injection capillary, and the material of the metal funnel were systematically optimized. The experimental results showed that when the filter paper with a pore size of 20-25 μm, the angle between the ion source tip and the horizontal plane is 15°-60°, and the distance between the ion source and the mass spectrometry injection capillary is 7.5 mm, the signal intensity is the highest, the used different metal materials have little effect on the signal intensity of the ion source. Under the optimized experimental conditions, standard samples of sucrose, kemptide, vitamin B2, rocuronium, arginine and fentanyl were tested, which greatly expanded the application range of the ion source. Analysis and detection of ammonia plus Huangmin capsules could quickly obtain the molecular ion peak of paracetamol (m/z 152), and the malachite green (m/z 329) molecular ion peak could be quickly detected in the living fish. In summary, the electric-filter paper spray ion source can realize rapid real-time detection and analysis, and reduce the complicated pre-processing process of the analytes. Furthermore, it can improve the sample analysis speed, and expand the application field of ion trap mass spectrometry technology. © 2021, Editorial Board of Journal of Chinese Mass Spectrometry Society. All right reserved.
引用
收藏
页码：381 / 389
页数：8
相关论文
共 25 条
  • [1] NIEHAUS M, SOLTWISCH J, BELOV M E, DREISEWERD K., Transmission-mode MALDI-2 mass spectrometry imaging of cells and tissues at subcellular resolution, Nature Methods, 16, 9, pp. 925-931, (2019)
  • [2] DONNELLY D P, RAWLINS C M, DEHART C J, FORNELLI L, AGAR J N., Best practices and benchmarks for intact protein analysis for top-down mass spectrometry, Nature Methods, 16, 7, pp. 587-594, (2019)
  • [3] SCHULZ S, BECKER M, GROSECLOSE M R, SCHADT S, HOPF C., Advanced MALDI mass spectrometry imaging in pharmaceutical research and drug development, Current Opinion in Biotechnology, 55, pp. 51-59, (2019)
  • [4] HARISCHANDRA N R, PALLAVI M S, BHEEMANNA M, PAVANKUMAR K, REDDY V C S, UDAYKUMAR N R, PARAMASIVAM M, YADAV S., Simultaneous determination of 79 pesticides in pigeonpea grains using GC-MS/MS and LC-MS/MS[J], Food Chemistry, 6, pp. 128-986, (2021)
  • [5] TAKATS Z, WISEMAN J M, GOLOGAN B, COOKS R G., Mass spectrometry sampling under ambient conditions with desorption electrospray ionization, Science, 306, 5 695, pp. 471-473, (2004)
  • [6] CODY R B, LARAMEE J A, DURST H D., Versatile new ion source for the analysis of materials in open air under ambient conditions, Analytical Chemistry, 77, 8, pp. 2297-2302, (2005)
  • [7] NA N, ZHANG C, ZHAO M, ZHANG S, YANG C, FANG X, ZHANG X., Direct detection of explosives on solid surfaces by mass spectrometry with an ambient ion source based on dielectric barrier discharge, Journal of Mass Spectrometry, 42, 8, pp. 1079-1085, (2007)
  • [8] CHEN H, VENTER A, COOKS R G., Extractive electrospray ionization for direct analysis of undiluted urine, milk and other complex mixtures without sample preparation, Chemical Communications, 19, pp. 2042-2044, (2006)
  • [9] LIU J, WANG H, MANICKE N E, LIN J, COOKS R G, OUYANG Z., Development, characterization, and application of paper spray ionization[J], Analytical Chemistry, 82, 6, pp. 2463-2471, (2010)
  • [10] GOMEZ-RIOS G A, PAWLISZYN J., Development of coated blade spray ionization mass spectrometry for the quantitation of target analytes present in complex matrices[J], Angewandte Chemie, 126, 52, pp. 14731-14735, (2014)
123