Characteristics of iron-sulfur metabolism and acid-producing microorganisms in groundwater contaminated by acid mine drainage in closed coal mines

The generation of acid mine drainage (AMD) from mining activities poses significant challenges to humanity due to its intense pollution and difficult management. Despite the hostile conditions presented by AMD, a plethora of microorganisms thrive in this environment. In this study, we employ metagenomic techniques and microbial diversity analysis to systematically categorize microorganisms. Specifically, we identify Sulfur-iron metabolizing microorganisms associated with AMD, encompassing 6 phyla and 1149 genera. Functional predictions and pathogenicity analyses are conducted on this microbial community. Furthermore, we specifically emphasize the study of acid-producing microorganisms. This research marks the first discussion on the scope and structure of acid-producing microorganisms., comprising 1 phylum, 39 genera, and 1393 species. Our research outcomes comprehensively depict the microbial diversity in AMD, unveiling their influence on the physicochemical properties of AMD pollution. This study contributes valuable insights to addressing environmental challenges arising from AMD and aids in its remediation.