Metabolic imaging in living plants: A promising field for chemical exchange saturation transfer (CEST) MRI

被引:0
作者
Mayer, Simon [1 ,2 ]
Rolletschek, Hardy [1 ]
Radchuk, Volodymyr [1 ]
Wagner, Steffen [1 ]
Ortleb, Stefan [1 ]
Guendel, Andre [3 ]
Dehmer, Klaus J. [1 ]
Gutjahr, Fabian T. [2 ]
Jakob, Peter M. [2 ]
Borisjuk, Ljudmilla [1 ]
机构
[1] Leibniz Inst Plant Genet & Crop Plant Res IPK, Corrensstr 3, D-06466 Seeland Gatersleben, Germany
[2] Univ Wurzburg, Inst Expt Phys 5, D-97074 Am Hubland, Germany
[3] Stockholm Univ, Dept Ecol Environm & Plant Sci, SE-10691 Stockholm, Sweden
来源
SCIENCE ADVANCES | 2024年 / 10卷 / 38期
关键词
ACCUMULATION; SPILLOVER; INSIGHTS; AGENTS; WATER;
D O I
10.1126/sciadv.adq4424
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Magnetic resonance imaging (MRI) is a versatile technique in the biomedical field, but its application to the study of plant metabolism in vivo remains challenging because of magnetic susceptibility problems. In this study, we report the establishment of chemical exchange saturation transfer (CEST) for plant MRI. This method enables noninvasive access to the metabolism of sugars and amino acids in complex sink organs (seeds, fruits, taproots, and tubers) of major crops (maize, barley, pea, potato, sugar beet, and sugarcane). Because of its high signal detection sensitivity and low susceptibility to magnetic field inhomogeneities, CEST analyzes heterogeneous botanical samples inaccessible to conventional magnetic resonance spectroscopy. The approach provides unprecedented insight into the dynamics and distribution of sugars and amino acids in intact, living plant tissue. The method is validated by chemical shift imaging, infrared microscopy, chromatography, and mass spectrometry. CEST is a versatile and promising tool for studying plant metabolism in vivo, with many applications in plant science and crop improvement.
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页数:10
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