Preparation and function of BRL-encapsulated poly-L-ornithine microcapsule

被引:0
作者
Liu, Yuan-Gang [1 ]
Bai, Yan [1 ]
Wang, Shi-Bin [1 ]
机构
[1] College of Chemical Engineering, Huaqiao University
来源
Gongneng Cailiao/Journal of Functional Materials | 2013年 / 44卷 / 12期
关键词
BRL cell line; Cell function; Microcapsule; Poly-L-ornithine;
D O I
10.3969/j.issn.1001-9731.2013.12.029
中图分类号
学科分类号
摘要
To investigate the influence of poly-L-ornithine on the functions of microencapsulated cells, in this article, BRL-encapsulated poly-L-ornithine/alginate microcapsules were prepared, and the cell viability and the cell functions were studied. The results showed that the BRL-encapsulated poly-L-ornithine microcapsules had excellent integrity and smooth surface. The BRL cells maintained cell viability and the functions to secrete urea and protein in the 3D microenvironment of poly-L-ornithine microcapsules, and it would supply experimental data in the future application of poly-L-ornithine as new membrane material in the filed of cell microencapsulation.
引用
收藏
页码:1804 / 1807
页数:3
相关论文
共 20 条
[1]  
Lim F., Sun A.M., Microencapsulated islets as bioartificial endocrine pancreas, Science, 210, pp. 908-910, (1980)
[2]  
Yu W., Song H., Zheng G., Et al., Study on membrane characteristics of alginate-chitosan microcapsule with cell growth, Journal of Membrane Science, 377, pp. 214-220, (2011)
[3]  
Kerby A., Jones E.S., Jones P.M., Et al., Co-transplantation of islets with mesenchymal stem cells in microcapsules demonstrates graft outcome can be improved in an isolated-graft model of islet transplantation in mice, Cytotherapy, 15, pp. 192-200, (2013)
[4]  
Pan X.P., Li L.J., Advances in cell sources of hepatocytes for bioartificial liver, Hepatobiliary & Pancreatic Diseases International, 11, pp. 594-605, (2012)
[5]  
Selimoglu S.M., Ayyildiz-Tamis D., Gurhan I.D., Et al., Purification of alginate and feasible production of monoclonal antibodies by the alginate-immobilized hybridoma cells, Journal of Bioscience and Bioengineering, 113, pp. 233-238, (2012)
[6]  
Lin Y.H., Yang Y.W., Chen Y.D., Et al., The application of an optically switched dielectrophoretic (ODEP) force for the manipulation and assembly of cell-encapsulating alginate microbeads in a microfluidic perfusion cell culture system for bottom-up tissue engineering, Lab Chip, 12, pp. 1164-1173, (2012)
[7]  
Wang S.B., Liu Y.G., Weng L.J., Et al., Effect of molecular weights of poly-l-arginine on membrane strength and permeating property of poly-l-arginine group microcapsule, Macromolecular Bioscience, 3, pp. 347-350, (2003)
[8]  
40, pp. 119-122, (2009)
[9]  
Darrabie M.D., Kendall Jr.W.F., Opara E.C., Characteristics of poly-L-ornithine-coated alginate microcapsules, Biomaterials, 26, pp. 6846-6852, (2005)
[10]  
Tam S.K., Bilodeau S., Dusseault J., Et al., Biocompatibility and physicochemical characteristics of alginate-polycation microcapsules, Acta Biomaterialia, 7, pp. 1683-1692, (2011)