Enantioseparation of amino acids and drugs by CEC, pressure supported CEC, and micro-HPLC using a teicoplanin aglycone stationary phase

Chiral separation of amino acids, drugs with amino alcohol structure, and other drugs has been investigated by capillary electrochromatography (CEC), pressure supported CEC, and micro-HPLC on a teicoplanin aglycone stationary phase. The teicoplanin aglycone lacks the D-glucosamine and the D-mannose moieties and shows different separation behavior compared to a teicoplanin stationary phase. The selector is bonded to 3.5-μm silica gel and separations are carried out either in the reversed phase or the polar organic mode. This chiral stationary phase (CSP) shows high enantioselectivity especially for amino acids, e.g. for DOPA a Rs value of 10.5 was achieved. Although the chiral recognition ability for drugs is lower than that of intact teicoplanin, out of 22 β-blockers investigated, 16 are baseline separated by CEC.