Sonoporation at a low mechanical index

被引：41
作者：
Delalande A. [1 ]
Kotopoulis S. [2 ]
Rovers T. [3 ]
Pichon C. [1 ]
Postema M. [1 ,2 ,3 ,4 ]
机构：
[1] Centre de Biophysique Moléculaire, UPR 4301 CNRS affiliated to the University of Orléans, 45071 Orléans Cedex 2, Rue Charles Sadron
[2] Department of Engineering, The University of Hull, Kingston upon Hull HU6 7RX, Cottingham Road
[3] Emmy Noether Research Group, Institute of Medical Engineering, Department of Electrical Engineering and Information Sciences, Ruhr-Universität Bochum, ID 04/24
[4] Department of Physics and Technology, University of Bergen, 5007 Bergen
来源：
Bubble Science, Engineering and Technology | 2011年 / 3卷 / 01期
基金：
英国工程与自然科学研究理事会;
关键词：
Cell penetration; Hela cells; Low mechanical index; Microbubbles; Sonoporation; Ultrasound contrast agent;
D O I：
10.1179/1758897911Y.0000000001
中图分类号：
学科分类号：
摘要：
The purpose of this study was to investigate the physical mechanisms of sonoporation, in order to understand and improve ultrasound-assisted drug and gene delivery. Sonoporation is the transient permeabilisation and resealing of a cell membrane with the help of ultrasound and/or an ultrasound contrast agent, allowing for the trans-membrane delivery and cellular uptake of macromolecules between 10 kDa and 3 MDa. The authors studied the behaviour of ultrasound contrast agent microbubbles near cancer cells at low acoustic amplitudes. After administering an ultrasound contrast agent, HeLa cells were subjected to 6.6 MHz ultrasound with a mechanical index of 0.2 and observed with a high-speed camera. Microbubbles were seen to enter cells and rapidly dissolve. The quick dissolution after entering suggests that the microbubbles lose (part of) their shell while entering. The authors have demonstrated that lipid-shelled microbubbles can be forced to enter cells at a low mechanical index. Hence, if a therapeutic agent is added to the shell of the bubble or inside the bubble, ultrasound-guided delivery could be facilitated at diagnostic settings. In addition, these results may have implications for the safety regulations on the use of ultrasound contrast agents for diagnostic imaging. © 2011 W. S. Maney & Son Ltd.
引用
收藏
页码：3 / 11
页数：8
相关论文
共 36 条
  • [1] Postema M., Gilja O.H., Ultrasound-directed drug delivery, Curr. Pharm. Biotechnol, 8, 6, pp. 355-361, (2007)
  • [2] Bao S., Thrall B.D., Miller D.L., Transfection of a reporter plasmid into cultured cells by sonoporation in vitro, Ultrasound Med. Biol, 23, pp. 953-959, (1997)
  • [3] Chen S., Shohet R.V., Bekeredjian R., Frenkel P., Grayburn P.A., Optimization of ultrasound parameters for cardiac gene delivery of adenoviral or plasmid deoxyribonucleic acid by ultrasound-targeted microbubble destruction, J. Am. Coll. Cardiol, 42, 2, pp. 301-308, (2003)
  • [4] Greenleaf W.J., Bolander M.E., Sarkar G., Goldring M.B., Greenleaf J.F., Artificial cavitation nuclei significantly enhance acoustically induced cell transfection, Ultrasound Med. Biol, 24, 4, pp. 587-595, (1998)
  • [5] Kondo I., Ohmori K., Oshita A., Takeuchi H., Fuke S., Shinomiya K., Noma T., Namba T., Kohno M., Treatment of acute myocardial infarction by hepatocyte growth factor gene transfer: The first demonstration of myocardial transfer of a ''functional'' gene using ultrasonic microbu-bble destruction, J. Am. Coll. Cardiol, 44, 3, pp. 644-653, (2004)
  • [6] Kudo N., Okada K., Yamamoto K., Sonoporation by single-shot pulsed ultrasound with microbubbles adjacent to cells, Biophys. J, 96, 12, pp. 4866-4876, (2009)
  • [7] Lindner J.R., Kaul S., Delivery of drugs with ultrasound, Echocardiography, 18, 4, pp. 329-337, (2001)
  • [8] Tachibana K., Uchida T., Ogawa K., Yamashita N., Tamura K., Induction of cell-membrane porosity by ultrasound, Lancet, 353, (1999)
  • [9] Tinkov S., Bekeredjian R., Winter G., Coester C., Microbubbles as ultrasound triggered drug carriers, J. Pharm. Sci, 98, 6, pp. 1935-1961, (2009)
  • [10] Klibanov A.L., Targeted delivery of gas-filled microspheres, contrast agents for ultrasound imaging, Adv. Drug Deliv. Rev, 37, pp. 139-157, (1999)
1234