Assembled bovine serum albumin onto titanium surface for improving the blood platelet adhesive behavior

The bovine serum albumin was assembled onto the titanium surface by a layer-by-layer self-assembly (LBL) technique for improving the blood compatibility of titanium. Titanium was first treated by NaOH solution for obtaining negative charge and pores surface with activity. Then, the sample was immersed into poly-L-lysine (PLL) solution for changing surface negative charge into positive charge. Finally, the sample was soaked in the bovine serum albumin (BSA) solution with negative charge for assembling BSA to titanium surface. The surface topography, the chemical composition and the wettability were investigated by scanning electron microscope (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR) and water contact angle measurement, respectively. The blood platelet adhesive behavior was evaluated by in vitro platelet adhesion experiment. The experiment result proved that the platelet adhesion had been well-controlled. The assembled titanium surface possesses the improving blood-compatibility.