Effect of microencapsulation on survival and stability of Bifidobacterium bifidum BB01 exposed to simulated gastrointestinal conditions and in different food matrices

被引:0
作者
Chen H. [1 ]
Donglin M.A. [1 ]
Li Y. [2 ]
Liu Y. [2 ]
Wang Y. [1 ]
机构
[1] School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an
[2] Shaanxi Heshi Dairy Co. Ltd., Baoji
关键词
Bifidobacterium bifidum BB01; Simulated gastrointestinal; Stability;
D O I
10.1515/aucft-2017-0003
中图分类号
学科分类号
摘要
Bifidobacterium bifidum BB01 was microencapsulated by extrusion method with sodium alginate. This paper aimed to study the survival rate of microencapsulating Bifidobacterium bifidum BB01 in simulated gastrointestinal conditions, resistance to artificial bile salt and stability during storage. Results showed non-microencapsulated Bifidobacterium bifidum BB01 were more susceptible than microencapsulated under simulated gastrointestinal conditions, and microencapsulated Bifidobacterium bifidum BB01 exhibited a lower population reduction than free cells during exposure to simulated gastrointestinal, The enteric test showed that the microorganism cells were released from the monolayer microcapsules, double microcapsules and trilayer completely in 40min. The viable counts of monolayer microcapsules, double layer microcapsules and triple layer microcapsules decreased by nine magnitudes, four magnitude and one magnitude after 2h, respectively. Moreover, in fruit orange, pure milk and nutrition Express, the optimum storage times of free Bifidobacterium bifidum BB01, monolayer microcapsules, double layer microcapsules and triple layer microcapsules were 21 days, 21 days, 28 days and more than 35 days at 4°C, but at room temperature the optimum storage time were 7 days, 14 days, 21 days and more than 28 days, and the viable counts were maintained at 1×106 CFU g-1 or more.
引用
收藏
页码:23 / 34
页数:11
相关论文
共 38 条
[1]  
Akalin A.S., Erisir D., Effects of inulin and oligofructose on the rheological characteristics and probiotic culture survival in low-fat probiotic ice cream, Food Sci, 73, pp. M184-M188, (2008)
[2]  
Albertini B., Vitali B., Passerini N., Cruciani F., Di Sabatino M., Rodriguez L., Brigidi P., Development of microparticulate systems for intestinal delivery of Lactobacillus acidophilus and Bifidobacterium lactis, European Journal of Pharmaceutical Sciences, 40, pp. 359-366, (2010)
[3]  
Anal A.K., Singh H., Recent advances in microencapsulation of probiotics for industrial applications and targeted delivery, Trends in Food Science and Technology, 18, pp. 240-251, (2007)
[4]  
Annan N.T., Borza A.D., Truelstrup L.H., Encapsulation in alginate-coated gelatin microspheres improves survival of the probiotic Bifidobacterium adolescentis 15703T during exposure to simulated gastro-intestinal conditions, Food Research Internationa, 41, 2, pp. 184-193, (2008)
[5]  
Aragon-Alegro L.C., Alegro J.H.A., Cardarelli H.R., Ming C.C., Saad S.M.I., Potentially probiotic and synbiotic chocolate mousse, LWT-Food Science and Technology, 40, 4, pp. 669-675, (2007)
[6]  
Borges J.Q., Ferreira S.R.S.S., Costa G.W., Cinética de sobrevivência de Lactobacillus acidophilus microencapsulados em matriz de alginato de cálcio eveiculados em musse de chocolate, In: Congresso Brasileiro de Ciência e Tecnologia de Alimentos, 19, (2004)
[7]  
Castro-Cislaghi F.P., Silva C.D.R.E., Fritzen-Freire C.B., Lorenz J.G.D., Sant'Anna E.S., Bifidobacterium, bb-12 microencapsulated by spray drying with whey: survival under simulated gastrointestinal conditions, tolerance to nacl, and viability during storage, Journal of Food Engineering, 113, 2, pp. 186-193, (2012)
[8]  
Chaikham P., Stability of probiotics encapsulated with thai herbal extracts in fruit juices and yoghurt during refrigerated storage, Food Bioscience, 12, pp. 61-66, (2015)
[9]  
Chandramouli V., Kailasapathy K., Peiris P., Jones M., An improved method of microencapsulation and its evaluation to protect Lactobacillus spp, In simulated gastric conditions. Journal of Microbiological Methods, 56, pp. 27-35, (2004)
[10]  
Chen C., Yang J., Zhu J.P., Sun Y.X., Research progress of wall materials and methods of probiotic microcapsule, Science and technology of food industry, 33, 14, pp. 403-407, (2012)