Microtubule-associated protein, MAP1B, , encodes functionally distinct polypeptides

Microtubule-associated protein, MAP1B, is crucial for neuronal morphogenesis and disruptions in MAP1B function are correlated with neurodevelopmental disorders. MAP1B encodes a single polypeptide that is processed into discrete proteins, a heavy chain (HC) and a light chain (LC); however, it is unclear if these two chains operate individually or as a complex within the cell. In vivo studies have characterized the contribution of MAP1B HC and LC to microtubule and actin- based processes, but their molecular mechanisms of action are unknown. Using in vitro reconstitution with purified fi ed proteins, we dissect the biophysical properties of the HC and LC and uncover distinct binding behaviors and functional roles for these MAPs. Our biochemical assays indicate that MAP1B HC and LC do not form a constitutive complex, supporting the hypothesis that these proteins operate independently within cells. Both HC and LC inhibit the microtubule motors, kinesin3, kinesin-4, and dynein, and differentially affect the severing activity of spastin. Notably, MAP1B LC binds to actin fi laments in vitro and can simultaneously bind and cross-link actin fi la- ments and microtubules, a function not observed for MAP1B HC. Phosphorylation of MAP1B HC by dual-specificity, fi city, tyrosine phosphorylation-regulated kinase 1a negatively regulates its actin-binding activity without significantly fi cantly affecting its microtubule-binding capacity, suggesting a dynamic contribution of MAP1B HC in cytoskeletal organization. Overall, our study provides new insights into the distinct functional properties of MAP1B HC and LC, underscoring their roles in coordinating cytoskeletal networks during neuronal development.