Fourteen train numbers in operation were selected from five vehicle types of Beijing-Shanghai and Wuhan-Guangzhou passenger dedicated lines. Such indexes as the interior thermal environment, air quality, transient pressure, noise, vibration and illumination during the peak passenger flow period in winter and summer were tested. Meanwhile, a questionnaire survey on ride comfort was conducted among 6210 passengers. According to passengers' feelings and the evaluation of interior environment, the technical conditions for the interior environmental of high speed EMU were studied. Results showed that the priority for the concerns of high speed EMU passengers on the influencing factors of their ride comfort were noise, transient pressure, undesirable odor, toilet facilities, temperature, vibration, seat width, air freshness, foot space and vehicle interior cleanliness. The appropriate technical conditions for the interior environment of high speed EMU are suggested as follows. The interior temperature in winter should be 22~24℃ in North China and 19~22℃ in South China, while in summer, it should be 25~27℃ in North China and 26~28℃ in South China. The air quality inside should be: CO2≤0.15%, TVOC 0.8~1 mg·m-3, HCHO≤0.1 mg·m-3, NH3≤0.2 mg·m-3, PM10≤0.15 mg·m-3, CO≤5 mg·m-3, total bacterial count≤2 500 cfu·m-3, O2≥20% and negative ion≥300 N·cm-3. The fresh air volume per person should be 30 m3·h-1 and 25 m3·h-1 for the first class and the second class cars respectively. Thus the comprehensive evaluation index of the air quality can reach level 2. The maximum change rate of the interior transient pressure in plain area should be 0.3 kPa·s-1 and 0.8 kPa·(3 s)-1, in mountainous area should be 0.2 kPa·s-1 and 0.5·(3 s)-1. The interior noise should be less than or equal to 65 dB(A) and 68 dB(A) respectively in the first class car and the second class car when the train speed is less than or equal to 250 km·h-1, while less than or equal to 68 dB(A) and 70 dB(A) respectively in the first class car and the second class car when the train speed is greater than or equal to 250 km·h-1. The interior vertical, longitudinal and lateral vibration acceleration should be less than or equal to 0.50, 0.38 and 0.38 m·s-2 respectively and the vibration acceleration vector should be less than or equal to 0.9 m·s-2. The interior illumination should be greater than or equal to 200 lx for the first class car and cushioned car, and greater than or equal to 150 lx for the second class car. ©, 2015, Chinese Academy of Railway Sciences. All right reserved.