Autofluorescence of chloroplasts measured by a laser scanning confocal microscope

被引：0
|
作者：
Feng Q. [1 ,2 ]
Wang W. [1 ,3 ]
Li H. [1 ,2 ]
Pan X. [1 ,2 ]
机构：
[1] School of Life Sciences, Tsinghua University, Beijing
[2] Center of Biomedical Analysis, Tsinghua University, Beijing
[3] Technology Center for Protein Sciences, Tsinghua University, Beijing
来源：
Pan, Xun (panx@biomed.tsinghua.edu.cn) | 1600年 / Tsinghua University卷 / 57期
关键词：
Autofluorescence; Chloroplast; Laser scanning confocal microscopy; Spectral imaging;
D O I：
10.16511/j.cnki.qhdxxb.2017.26.034
中图分类号：
学科分类号：
摘要：
The autofluorescence of nicotiana benthamiana chloroplasts was studied using a laser scanning confocal microscope. The chloroplast autofluorescence was obtained by spectral scanning with various excitation wavelengths. Then, chloroplast autofluorescence images were obtained using laser scanning confocal microscopy with various excitation wavelengths with intensity quantification. Finally, nicotiana benthamiana chloroplasts were illuminated with 488 nm light to test the autofluorescence stability. 488 nm light has the highest excitation efficiency for chloroplast autofluorescence, while 561 nm light has the lowest excitation efficiency. The autofluorescence chloroplast emission spectrum starts from 637 nm and peaks at 681 nm. The measurements show that the chloroplast autofluorescence is very stable and that laser scanning confocal microscopy can accurately measure chloroplasts autofluorescence. This work will facilitate improved plant tissue imaging chloroplast genetic engineering research. © 2017, Tsinghua University Press. All right reserved.
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页码：651 / 654and660
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