Identification and nitrogen removal characteristics of a heterotrophic nitrification-aerobic denitrification strain isolated from marine environment

被引:0
|
作者
Sun Q.-H. [1 ]
Yu D.-S. [1 ]
Zhang P.-Y. [1 ]
Lin X.-Z. [2 ]
Li J. [1 ]
机构
[1] School of Environmental Science and Engineering, Qingdao University, Qingdao
[2] Key Laboratory of Marine Bioactive Substances, First Institute of Oceanography, State Oceanic Administration, Qingdao
来源
Huanjing Kexue/Environmental Science | 2016年 / 37卷 / 02期
关键词
Aerobic denitrification; Characteristics of nitrogen removal; Heterotrophic nitrification; Heterotrophic nitrification-aerobic denitrification system; Klebsiella sp; Marine environments;
D O I
10.13227/j.hjkx.2016.02.033
中图分类号
学科分类号
摘要
A heterotrophic nitrification-aerobic denitrification strain named y5 was isolated from marine environment by traditional microbial isolation method using seawater as medium. It was identified as Klebsiella sp. based on the morphological, physiological and 16S rRNA sequence analysis. The experiment results showed that the optimal carbon resource was sodium citrate; the optimal pH was 7.0; and the optimal C/N was 17.The strain could use NH4Cl, NaNO2 and KNO3 as sole nitrogen source, and the removal efficiencies were 77.07%, 64.14% and 100% after 36 hours, respectively. The removal efficiency reached 100% after 36 hours in the coexistence of NH4Cl, NaNO2 and KNO3. The results showed that the strain y5 had independent and efficient heterotrophic nitrification and aerobic denitrification activities in high salt wastewater. © 2016, Science Press. All right reserved.
引用
收藏
页码:647 / 654
页数:7
相关论文
共 9 条
  • [1] Robertson L.A., Kuenen J.G., Thiosphaera pantotropha gen. nov. sp. nov., a facultatively anaerobic, facultatively autotrophic sulphur bacterium, Journal of General Microbiology, 129, 9, pp. 2847-2855, (1983)
  • [2] Wan C.L., Yang X., Lee D.J., Et al., Aerobic denitrification by novel isolated strain using NO<sub>2</sub><sup>-</sup>-N as nitrogen source, Bioresource Technology, 102, 15, pp. 7244-7248, (2011)
  • [3] Yao S., Ni J.R., Chen Q., Et al., Enrichment and characterization of a bacteria consortium capable of heterotrophic nitrification and aerobic denitrification at low temperature, Bioresource Technology, 127, pp. 151-157, (2013)
  • [4] Zhang J.B., Wu P.X., Hao B., Et al., Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001, Bioresource Technology, 102, 21, pp. 9866-9869, (2011)
  • [5] Shapovalova A.A., Khijniak T.V., Tourova T.P., Et al., Heterotrophic denitrification at extremely high salt and pH by haloalkaliphilic Gammaproteobaeteria from hypersaline soda lakes, Extremophiles, 12, 5, pp. 619-625, (2008)
  • [6] Gupta A.B., Thiosphaera pantotropha: a sulphur bacterium capable of simultaneous heterotrophic nitrification and aerobic denitrification, Enzyme and Microbial Technology, 21, 8, pp. 589-595, (1997)
  • [7] Joo H.S., Hirai M., Shoda M., Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis No. 4, Journal of Bioscience and Bioengineering, 100, 2, pp. 184-191, (2005)
  • [8] Richardson D.J., Wehrfritz J.M., Keech A., Et al., The diversity of redox proteins involved in bacterial heterotrophic nitrification and aerobic denitrification, Biochemical Society Transactions, 26, 3, pp. 401-408, (1998)
  • [9] Stewart V., Nitrate respiration in relation in to facultative metabolism in enterobacteria, Microbiology Reviews, 52, 2, pp. 190-232, (1988)