Polypyrrole-based xanthine oxidase potentiometric biosensor for hypoxanthine

被引：11

作者：

Lawal, A.T. ^{[1
]}

Adeloju, S.B. ^{[2
]}

机构：

[1] School of Agriculture and Wine Science, Charles Sturt University, Wagga

[2] Water and Sensor Research Group, School of Applied Science and Engineering, Monash University, Churchill Vic 3842, Gippsland Campus

来源：

Journal of Applied Sciences | 2008年 / 8卷 / 14期

关键词：

Biosensor; Fish freshness; Hypoxanthine; Polypyrrole; Potentiometric and xanthine oxidase;

D O I：

10.3923/jas.2008.2599.2605

中图分类号：

学科分类号：

摘要：

A biosensor is developed for the determination of hypoxanthine (Hx) by entrapment of XOD and a mediator, ferrocene or potassium ferrocyanide, (K4Fe(CN)6, into a PPy film during galvanostatic film formation. The optimum conditions for the formation of PPy-XOD-Fe(CN)64- film include 0.4 M pyrrole, 6.2 U mL-1 XOD, 40 mM K4Fe(CN)6, polymerisation period of 200 sec and an applied current density of 0.5 mA cm-2. Potentiometric modes of detection, was investigated. The potential for the potentiometric biosensing of Hx was 0.0 mV vs. Ag/AgCI (3 M KCl) in 0.05 M phosphate buffer. It is revealed that potentiometric detection was sensitive and gave a wider linear concentration range. The PPy-XOD potentiometric biosensor was successfully used for the determination of fish freshness. © 2008 Asian Network for Scientific Information.

引用

页码：2599 / 2605

页数：6

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