The food-grade cell wall anchored high efficient induction expression the fusion protein S of TGEV and PEDV in lactococcus lactis and the immunogenicity analysis

被引：0

作者：

Xu, Bo ^{[1
]}

Xiong, Weina ^{[1
]}

Li, Yeqing ^{[1
]}

Zhou, Tong ^{[1
]}

Liu, Zhiwen ^{[1
]}

Ying, Bi ^{[1
]}

Guo, Xiaoyan ^{[1
]}

机构：

[1] Biology Science and Engineering College, Jiangxi Agricultural University, Nanchang

来源：

Journal of Chinese Institute of Food Science and Technology | 2015年 / 15卷 / 06期

关键词：

Cell wall anchored high efficient induction expression; Food-grade vector; Fusion protein S of TGEV and PEDV; Immunogenicity; Lactococcus lactis;

D O I：

10.16429/j.1009-7848.2015.06.004

中图分类号：

学科分类号：

摘要：

Porcine transmissible gastroenteritis and porcine epidemic diarrhea are widely spread in China, which has brought huge economic losses to Chinese breeding industry. At present, the prevention and treatments of the two diseases are still using conventional vaccine. The existing vaccine is not effective enough and there is an urgent need for a safe and efficient means of prevention. Firstly, the fusion gene S of TGEV and PEDV was successfully constructed. The pRNV48-TPs were successfully constructed by using the food-grade cell wall anchored high efficient induced expression vector pRNV48 of Lactococcus lactis. To acquire the recombinant bacteria L. lactis NZ9000/pRNV48-TPs, the recombinant plasmid was transferred into the L. lactis NZ9000 competent cells by electroporation method. Then the recombinant strain was induced by nisin and the SDS-PAGE was used to analyse the expression of the target protein after the cell wall protein was extracted. And then rabbits were administrated by injection immunization and oral immunization respectively and then analyzed by Western Blot. In order to discuss the immune response mechanism of the oral vaccine and lay a good foundation for TGEV and PEDV research and development of a safe and efficient vaccine, the Lactococcus lactis food-grade cell wall anchored high efficient induced expression vector carrier was used to induce the fusion protein S of TGEV and PEDV to produce neutralizing antibodies. ©, 2015, Chinese Institute of Food Science and Technology. All right reserved.

引用

页码：32 / 38

页数：6

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