Inactivation of Bacillus subtilis spores in water with chlorine

被引：0
|
作者：
机构：
[1] State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, Heilongjiang
[2] State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences
来源：
Chen, Z. (blist@126.com) | 2013年 / Materials China卷 / 64期
关键词：
Bacillus anthracis spores; Bacillus subtilis spores; Chlorine; Inactivation;
D O I：
10.3969/j.issn.0438-1157.2013.08.040
中图分类号：
学科分类号：
摘要：
Spores of Bacillus subtilis(ATCC6633)could be used as a potential model for the resistant microorganisms. The inactivation effect of the spores in drinking water by chlorine was studied, and such factors as chlorine concentration, contact time, pH, temperature, and initial density of spores, which might influence the inactivation were evaluated. The results showed that the inactivation process of spores by chlorine was characterized by a lag phase and a logarithmic phase of inactivation. With chlorine concentration from 2.06 to 10.30 mg·L-1, reaction time from 0 to 166 min, pH from 6 to 9, temperature from 1 to 30°C, and initial density of spores from 102 to 1012cfu·ml-1, both chlorine concentration and reaction time influenced the inactivation effect of spores, and rate of inactivation would increase at either higher chlorine concentration or longer reaction time. The inactivation ability of chlorine was stronger under acidic condition than that under alkali condition. With increasing temperature, the inactivation ability of chlorine was enhanced, while initial density of spores nearly had no effect. This study indicated that Bacillus subtilis spores were more resistant to chlorine than Bacillus anthracis spores. © All Rights Reserved.
引用
收藏
页码：2982 / 2988
页数：6
相关论文
共 23 条
  • [1] Shannon M.A., Bohn P.W., Elimelech M.E., Georgiadis J.G., Marinas B.J., Mayes A.M., Science and technology for water purification in the coming decades, Nature, 452, 20, pp. 301-310, (2008)
  • [2] Stlow B., Mcginnis K.A., Ragkousi K., Setlow P., Effects of major spore-specific DNA binding proteins on Bacillus subtilis sporulation and spore properties, Bacteriol., 18, 24, pp. 6906-6912, (2000)
  • [3] Essam A.M., Gui-Su P., Choi I.W., Cho S., Kim H., Comparison of Fe(VI)(Fe) and ozone in inactivating Bacillus subtilis spores, Chemosphere, 83, 9, pp. 1228-1233, (2011)
  • [4] Erick R.B., Roberto P., Angel E.V.L., Jose L.S., Marco A.Q., Miguel A.M.R., Bacillus subtilis spore inactivation in water using photo-assisted Fenton reaction, Sustain. Environ. Res., 21, 5, pp. 285-290, (2011)
  • [5] Cho M., Gandhi V., Hwang T.M., Lee S., Kim J.H., Investigating synergism during sequential inactivation of MS-2 phage and Bacillus subtilis spores with UV/H<sub>2</sub>O<sub>2</sub> followed by free chlorine, Wat. Res., 45, 3, pp. 1063-1070, (2011)
  • [6] Craik S.A., Smith D.W., Belosevic M., Chandrakanth M., Use of Bacillus subtilis spores as model microorganisms for ozonation of Cryptosporidium parvum in drinking water treatment, J. Environ. Eng. Sci., 1, 3, pp. 173-186, (2002)
  • [7] Cho M., Kim J.H., Yoon J.Y., Investigating synergism during sequential inactivation of Bacillus subtilis spores with several disinfectants, Wat. Res., 40, 15, pp. 2911-2920, (2006)
  • [8] Barbeau B., Boulos L., Desjardins R., Coallier J., Prevost M., Examining the use of aerobic spore-forming bacteria to assess the efficiency of chlorination, Wat. Res., 33, 13, pp. 2941-2948, (1999)
  • [9] Khan A.S., Public-health preparedness for biological terrorism in the USA, Lancet, 356, 9236, pp. 1179-1182, (2000)
  • [10] Technical Guide 188: U. S. Army Food and Water Vulnerability Assessment Guide, (2008)
123