Cryopreservation and plant regeneration via somatic emberyogenesis in Clitoria ternatea Linn

被引：0

作者：

Malabadi, Ravindra B. ^{[1
,2
]}

Nataraja, K. ^{[1
]}

机构：

[1] Division of Plant Tissue Culture, Department of Botany, Karnatak University, Dharwad - 580 003, Karnataka, India

[2] H.No. 609, Teil - Patil - Galli, Belgaum - 590 003, Kamataka, India

来源：

Phytomorphology: An International Journal of Plant Morphology | 2004年 / 54卷 / 1-2期

关键词：

Acetic acid - Cells - Chemical modification - Freezing - Genetic engineering - Nitrogen - Sulfur compounds - Thermal effects;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Embryogenic callus of Clitoria ternatea (Linn.) was cryopreserved for 8 weeks using sorbitol and dimethylsulfoxide as cryoprotectants with two types of freezing procedures. Results indicate that preculture of embryogenic cells for 24hr with 0.2M sorbitol and 5% dimethylsulfoxide had the best cryoprotecting effect using stepwise and fast cooling freezing procedures. Recovered embryogenic cells produced somatic embryos which produced complete plantlets when transferred to half strength modified Murashige & Skoog (1962) (MS 3) for 3 to 4 weeks and half strength modified Murashige & Skoog basal medium (MS4) for 2 to 3 weeks respectively. Cryopreserved cultures with both freezing procedures produced embryos and had the same appearance as the untreated and unfrozen controls.

引用

页码：7 / 17