Dual-color FISH study on myelodysplastic syndrome with 1;7 translocation

Objective: To study the myelodysplastic syndrome (MDS) with 1;7 translocation in five cases and to determine further the constitution and origin of centromere of the derivative chromosome resulting from 1;7 translocation. Methods: Bone marrow chromosome preparation of five cases was made using direct method or short-term culture. Karyotypic analysis was carried out by R-banding technique. Dual-color fluorescence in situ hybridization (FISH) using SpectrumRed and SpectrumGreen directly labeled chromosome 1-specific a-satellite DNA probe (red) and chromosome 7-specific α-satellite DNA probe (green) was performed in three patients of them. Results: All of the five cases had 1;7 translocation. The centromere of the derivative chromosome 7p/1q was constituted with red and green signals in three of them. Conclusion: The result of dual-color FISH confirms that the centromere of the derivative chromosome resulting from 1;7 translocation originated from both centromeres of chromosome 1 and chromosome 7.