Hepatogenic differentiation of mesenchymal stem cells using microfluidic chips

被引：26

作者：

Ju, Xiuli ^{[1
]}

Li, Dong ^{[1
]}

Gao, Ning ^{[1
]}

Shi, Qing ^{[1
]}

Hou, Huaishui ^{[1
]}

机构：

[1] Cryomedicine Laboratory, Qilu Hospital, Shandong University, Jinan

来源：

Biotechnology Journal | 2008年 / 3卷 / 03期

关键词：

Cell culture; Differentiation; Mesenchymal stem cells; Microfluidic chip;

D O I：

10.1002/biot.200700152

中图分类号：

学科分类号：

摘要：

Directional induction and differentiation of mesenchymal stem cells (MSCs) is very important to clinical therapy, but the mechanisms that govern differentiation are not well understood. However, traditional plate culture cannot precisely control cellular behavior because cells take up substances while secreting cytokines and wastes. Here, we used a microfluidic device to culture MSCs inside a microchamber. Hepatic differentiation medium was perfused to evaluate the ability of MSCs to differentiate toward hepatic cells on the chip. Parallel differentiation on 96-well plates was used to provide a detailed comparison of the differences between the two culturing methods. After treatment for 4 weeks, differentiated cells from both groups could express hepatocyte-specific markers, including α-fetoprotein, tyrosine aminotransferase, and albumin. The bioactivity assays revealed that these hepatocyte-like cells could uptake lipoprotein, but cells that differentiated on the chip showed more positive signals than the cells cultured on plates. Our results indicated that a microfluidic platform might be a potential tool for cost-effective and automated cell culture, and have potential applications in reliable cell-based screens and assays. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：383 / 391

页数：8

共 25 条

[1]

Horwitz E.M., Gordon P.L., Koo W.K., Marx J.C., Et al., Isolated allogeneic bone marrow-derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: Implications for cell therapy of bone, Proc. Natl. Acad. Sci USA, 99, pp. 8932-8937, (2002)

[2]

Koc O.N., Gerson S.L., Cooper B.W., Dyhouse S.M., Et al., Rapid hematopoietic recovery after coinfusion of autologousblood stem cells and culture-expanded marrow mesenchymal stem cells in advanced breast cancer patients receiving high-dose chemotherapy, J. Clin. Oncol, 18, pp. 307-316, (2000)

[3]

Petite H., Viateau V., Bensaid W., Meunier A., Et al., Tissue-engineered bone regeneration, Nat. Biotechnol, 18, pp. 959-963, (2000)

[4]

Gage F.H., Mammalian neural stem cells, Science, 287, pp. 1433-1438, (2000)

[5]

Temple S., The developments of neural stem cells, Nature, 414, pp. 112-117, (2001)

[6]

Chung B.G., Flanagan L.A., Rhee S.W., Schwartz P.H., Et al., Human neural stem cell growth and differentiation in a gradient-generating microfluidec device, Lab Chip, 5, pp. 401-406, (2005)

[7]

Nie F.-Q., Yamada M., Kobayashi J., Yamato M., Et al., Onchip cell migration assay using microfluidic channels, Biomaterials, 28, pp. 4017-4022, (2007)

[8]

Jeon N.L., Baskaran H., Dertinger S.K.W., Whitesides G.M., Et al., Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed in a microfabricated device, Nat. Biotechnol, 20, pp. 826-830, (2002)

[9]

Wang S.J., Saadi W., Lin F., Nguyen C.M., Et al., Differential effects of EGF gradient profiles on MDA-MB-231 breast cancer cell chemotaxis, Exp. Cell Res, 300, pp. 180-189, (2004)

[10]

Gu W., Zhu X., Futai N., Cho B.S., Et al., Computerized microfluidic cell culture using elastomeric channels and Braille displays, Proc. Natl. Acad. Sci. USA, 101, pp. 15861-15866, (2004)

← 1 2 3 →