Comparison of DNA damage induced by different alkylating agent by single cell gel electrophoresis assay

被引：0

作者：

Chen, Xue-Chai ^{[1
,2
]}

Zheng, Xiao-Tong ^{[1
,2
]}

Cui, Bo ^{[1
,2
]}

Luo, Yun-Jing ^{[1
,2
]}

Zhong, Ru-Gang ^{[1
,2
]}

机构：

[1] College of Life Science and Bioengineering, Beijing University of Technology, Beijing

[2] Beijing Key Laboratory of Environment and Viral Oncology, Beijing

来源：

Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology | 2015年 / 35卷

关键词：

Alkylating agent; DNA crosslinking; DNA damage; Nimustine; SCGE;

D O I：

10.15918/j.tbit1001-0645.2015.supplement1.010

中图分类号：

学科分类号：

摘要：

Toxic effects of alkylating agents on cell survival and DNA damage was investigated on a single cell level. In this study, single cell gel electrophoresis assay (SCGE) was used to detect and compare DNA damages induced by monofunctional and bifunctional alkylating agents under different concentrations. Linear positive correlation between drug concentration and DNA damages has been demonstrated. To further investigate the possible induction of DNA crosslinking by ACNU, DNA cleavage reagent was used, and results show that ACNU can cause significant DNA crosslinks. The result have provided theoretical basis for clarifying carcinogenic and anti-cancer mechanisms of alkylating agents. ©, 2015, Beijing Institute of Technology. All right reserved.

引用

页码：45 / 49

页数：4

共 12 条

[1]

Huynh G.H., Deen D.F., Szoka F.J., Barriers to carrier mediated drug and gene delivery to brain tumors, J Control Release, 110, 2, pp. 236-259, (2006)

[2]

Johnson R.E., Yu S.L., Prakash S., Et al., A role for yeast and human translesion synthesis DNA polymerases in promoting replication through 3-methyl adenine, Mol Cell Biol, 27, 20, pp. 7198-7205, (2007)

[3]

Fu D., Calvo J.A., Samson L.D., Balancing repair and tolerance of DNA damage caused by alkylating agents, Nat Rev Cancer, 12, 2, pp. 104-120, (2012)

[4]

Loveless A., Possible relevance of O6-alkylation of deoxyguanosine to the mutagenicity and carcinogenicity of nitrosamines and nitrosamides, Nature, 223, 5202, pp. 206-207, (1969)

[5]

Kondo N., Takahashi A., Mori E., Et al., FANCD1/BRCA2 plays predominant role in the repair of DNA damage induced by ACNU or TMZ, PLOS One, 6, 5, (2011)

[6]

Hara A., Zhang W., Kobayashi H., Et al., A single cell gel electrophoresis technique for the detection of DNA damage induced by ACNU, an alkylating agent or irradiation in murine glioma cell lines, Neurol Res, 16, 4, pp. 234-240, (1994)

[7]

Zhao L., Chen X., Zhong Y., Et al., Effect of different breaking agents exposed order on DNA crosslinking detection, Journal of Anhui Agri Sci, 7, pp. 2848-2849, (2013)

[8]

Stephanou G., Vlastos D., Vlachodimitropoulos D., Et al., A comparative study on the effect of MNU on human lymphocyte cultures in vitro evaluated by O6-mdG formation, micronuclei and sister chromatid exchanges induction, Cancer Lett, 109, 1-2, pp. 109-114, (1996)

[9]

Dai Q., Lu P., Peng S., Et al., Aflatoxin and N-nitroso compounds cause cancer by inducing DNA cross-links between complementary base pairs, Progress in Natural Science, 7, pp. 23-27, (2003)

[10]

Huang Y., Li L., DNA crosslinking damage and cancer - A tale of friend and foe, Transl Cancer Res, 2, 3, pp. 144-154, (2013)

← 1 2 →