Metabolic Pathway Optimization of Methionine Biosynthesis Using Recombinant Escherichia Coli

被引：0
|
作者：
Gao H.-J. [1 ]
Yang Y.-F. [1 ]
Meng Q. [1 ]
机构：
[1] School of Life Science, Beijing Institute of Technology, Beijing
来源：
Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities | 2017年 / 31卷 / 04期
关键词：
Metabolism; Methionine; Methionine transport; Pathway optimization;
D O I：
10.3969/j.issn.1003-9015.2017.04.018
中图分类号：
学科分类号：
摘要：
L-methionine as a sulfur-containing amino acid has important physiological functions and application values. In order to improve methionine biosynthesis, feedback repression of methionine synthesis was partially alleviated by knocking out the encoding repressor protein gene metJ and the encoding homoserine kinase gene thrB. The results show that methionine accumulation can reach to 0.18 g·L-1 using the resulting strain. Based on the known functional genes related to methionine accumulation, synthesis and metabolism of methionine was optimized concerning methionine metabolism network and transportation by knockout and overexpression techniques. Effects of key steps in methionine pathway, methionine transportation, threonine synthesis pathway and methionine recycling pathway were also investigated, and the metabolic network of E. coli was optimized. Transportation of methionine to extracellular was enhanced. Methionine concentration can reach to 0.40 g·L-1 after 24 h cultivation of the optimized strain. This work is helpful in the improvement of methionine biosynthesis. © 2017, Editorial Board of “Journal of Chemical Engineering of Chinese Universities”. All right reserved.
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页码：884 / 891
页数：7
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