Optimization of culture medium for production of recombinant dengue protein in escherichia coli

被引：0

作者：

Tripathi, Nagesh K. ^{[1
,2
]}

Shrivastva, Ambuj ^{[1
]}

Biswal, Karttik C. ^{[2
]}

Rao, P.V. Lakshmana ^{[1
]}

机构：

[1] Bioprocess Scale-up Facility, Defence Research, Development Establishment, Gwalior-474002, Jhansi Road

[2] Department of Chemical Engineering, National Institute of Technology

来源：

Industrial Biotechnology | 2009年 / 5卷 / 03期

关键词：

Recombinant dengue protein;

D O I：

10.1089/ind.2009.3.179

中图分类号：

学科分类号：

摘要：

Enhanced production of recombinant dengue multi-epitope protein in Escherichia coli was achieved by optimization of culture medium. Complex media (Luria Bertani broth, Terrific broth, Super broth, M9 minimal media, five times Luria Bertani broth, semi-defined medium enriched with tryptone and yeast extract, and semi-defined medium enriched with glucose) were evaluated for production of recombinant dengue multi-epitope protein in shake flasks. The recombinant protein was further produced by fed-batch fermentation using 5 L bioreactor. Cells were grown in optimized semi-defined medium, and feeding was carried out with 5X medium and glycerol. When growth reached 14.35 g/L of dry cell weight, culture was induced with 0.5 mM IPTG and further grown for 4 h to reach 18.37 g/L dry cell weight. The recombinant dengue multi-epitope protein was purified from inclusion bodies under denaturing conditions using metal affinity chromatography, which yielded 96.43 mg/L of protein. The purified protein was found to be reactive with dengue-infected human serum samples. Copyright 2009, Gen Publishing Inc., Mary Ann Liebert, Inc.

引用

页码：179 / 183

页数：4