Antioxidant Activity of Oyster Peptide Fraction OE-I and Its Anti-aging Effect on Caenorhabditis elegans

被引：1

作者：

Wang L. ^{[1
,2
]}

Xiao M. ^{[1
,2
]}

Chen H. ^{[1
,2
]}

Liu B. ^{[1
,2
]}

Zeng F. ^{[1
,2
]}

机构：

[1] Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou

[2] College of Food Science, Fujian Agriculture and Forestry University, Fuzhou

来源：

Shipin Kexue/Food Science | 2022年 / 43卷 / 03期

关键词：

Anti-aging; Antioxidant; Caenorhabditis elegans; Daf-16; gene; Oyster peptides;

D O I：

10.7506/spkx1002-6630-20210201-016

中图分类号：

学科分类号：

摘要：

Oyster enzymatic hydrolysate (OE) was fractionated by ultrafiltration into three fractions: OE-I (< 5 kDa), OE-II (5-10 kDa) and OE-III (> 10 kDa).The in vitro antioxidant activities of the fractions were evaluated in terms of their 1, 1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and superoxide anion radical scavenging capacity and ferrous ion chelating capacity.Then, the effect of different doses of OE-I on the lifespan, acute oxidative stress, thermal stress, antioxidant enzyme activities, reactive oxygen species (ROS) accumulation and cell apoptosis of Caenorhabditis elegans was investigated, and the expression levels of lifespan-related genes were measured to unravel the anti-aging mechanism of OE-I.The results showed that OE-I had the strongest antioxidant activity among the three fractions, and that high-dose of OE-I significantly prolonged the lifespan of C.elegans (P < 0.01).The activities of superoxide dismutase (SOD) (P < 0.05), catalase (CAT) and glutathione peroxidase (GSH-Px) (P < 0.01) were significantly increased and the accumulation of ROS and cell apoptosis were decreased.In addition, the expression of the skn-1, daf-16, sod-3 and mtl-1 genes were up-regulated, while the expression of the daf-2 and age-1 genes were down-regulated, suggesting improved stress resistance of C.elegans.To sum up, OE-I can be used as a functional food base material for anti-oxidation and anti-aging. © 2022, China Food Publishing Company. All right reserved.

引用

页码：152 / 160

页数：8

共 30 条

[1]

GUEDJ A, VOLMAN Y, GEIGER-MAOR A, Et al., Gut microbiota shape ‘inflamm-ageing' cytokines and account for age-dependent decline in DNA damage repair, Gut, 69, 6, pp. 1064-1075, (2020)

[2]

HE Ming, CHIANG Houhsien, LUO Hanzhi, Et al., An acetylation switch of the NLRP3 inflammasome regulates aging-associated chronic inflammation and insulin resistance, Cell Metabolism, 31, 3, pp. 580-591, (2020)

[3]

4, pp. 62-65, (2004)

[4]

[5]

XIAO Meifang, LIN Luan, CHEN Hongpei, Et al., Anti-fatigue property of oyster polypeptide fraction and its effect on gut microbiota in mice, Food & Function, 11, pp. 8659-8669, (2020)

[6]

MIAO Jianyin, LIAO Wanwen, KANG Meng, Et al., Anti-fatigue and anti-oxidant activities of oyster (Ostrea rivularis) hydrolysate prepared by compound protease, Food & Function, 9, 12, pp. 6577-6585, (2018)

[7]

UMAYAPARVATHI S, MEENAKSHI S, VIMALRAJ V, Et al., Isolation and structural elucidation of antioxidant peptides from oyster (Saccostrea cucullata) protein hydrolysate, Protein & Peptide Letters, 21, 10, pp. 1073-1083, (2014)

[8]

pp. 46-47, (2015)

[9]

NIGON V M, FELIX M A., History of research on C.elegans and other free-living nematodes as model organisms, WormBook, 2017, pp. 1-91, (2017)

[10]

WATTS J L, RISTOW M., Lipid and carbohydrate metabolism in Caenorhabditis elegans, Genetics, 207, 2, pp. 413-446, (2017)

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