Selection and function of an anaerobic microbial community with raw cellulose degradation ability

被引：0

作者：

Yu, Yan-Ling ^{[1
]}

Feng, Yu-Jie ^{[1
]}

Gao, Fei ^{[1
]}

Yu, Li-Xin ^{[1
]}

Xu, Chen ^{[1
]}

机构：

[1] State Key Lab. of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China

来源：

Harbin Gongye Daxue Xuebao/Journal of Harbin Institute of Technology | 2011年 / 43卷 / 02期

关键词：

Enzyme activity - Biodegradation - Cellulose;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

An anaerobic microbial community cultured in vitro with efficient and stable ability of cellulose degradation was obtained from rumen residue by the techniques of restricted cultivation, which was named as FYG-2. The characteristics of FYG-2 for cellulose degradation were investigated by taking enzymic activity, gravimetry and glucose concentration as parameter. FYG-2 can hydrolyze 1.00 g filter paper completely within 5 d at the condition of 38.5°C and pH6.4-6.8, and degrade both non-sterilized and sterilized substrate and its degradation ability keeps stable after 30 generations. It can degrade natural lignocellulosic materials more easily than pretreated one. The highest enzyme activity (159.7 IU) was obtained with 15 percents initial seed ratio and after 3 d cultivation. Seven pure cultures were separated from FYG-2, however, all their enzymic activities were far lower than FYG-2. This phenomenon demonstrated that strong synergy probably existed in the cellulose-degrading microorganisms and non-cellulose-degrading microorganisms or in the cultured microorganisms and uncultured microorganisms.

引用

页码：30 / 34