Identification and localization of a novel ecdysone oxidase in the silkworm, bombyx mori

被引：0
|
作者：
Yamamoto K. [1 ]
Nagaoka S. [2 ,3 ]
机构：
[1] Department of Bioscience and Biotechnology, Kyushu University Graduate School, Fukuoka
[2] Department of Applied Biology, Kyoto Institute of Technology, Kyoto
[3] The Center for Advanced Insect Research Promotion (CAIRP), Kyoto Institute of Technology, Kyoto
来源：
Yamamoto, Kohji (yamamok@agr.kyushu-u.ac.jp) | 1600年 / Japanese Society of Sericultural Sciences卷 / 86期
基金：
日本学术振兴会;
关键词：
Bombyx mori; CDNA sequence; Ecdysone; Ecdysone oxidase; Silkworm;
D O I：
10.11416/jibs.86.2_049
中图分类号：
学科分类号：
摘要：
Ecdysone oxidase (EO) is an enzyme that catalyzes the oxidation of ecdysteroids, the molting steroid hormones in insects. This enzyme may play important roles in the conversion of ecdysteroids to 3-dehydroecdyster-oids. In this study, we identified a novel EO, designated bmEO2, from the silkworm, Bombyx mori. A cDNA encoding bmEO2 was cloned by employing the reverse transcriptase-polymerase chain reaction (PCR) tech-nique. The deduced amino acid sequence of bmEO2 showed conserved residues and sequence homology to other known insect EOs and glucose dehydrogenase proteins. Quantitative real-time PCR revealed that the bmEO2 transcript was abundantly present in larval testes of B. mori. © 2017, Japanese Society of Sericultural Sciences. All rights reserved.
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页码：49 / 53
页数：4
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