Comparison of tobacco mosaic virus strains isolated from tobacco grown in different areas in Yunnan Province

被引：0

作者：

机构：

[1] Key Laboratory of Yunnan Agricultural Biotechnology, Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming, 650223, Yunnan

[2] Yunnan Academy of Tobacco Agricultural Research, Yuxi, 653100, Yunnan

来源：

Ding, M. (mingd73@163.com) | 1600年 / Acta Tabacaria Sinica Editorial Department, 55 Yuetan South Street, Beijing, 100045, China卷 / 19期

关键词：

Strain; Tobacco mosaic virus; Tobacco plant;

D O I：

10.3969/j.issn.1004-5708.2013.06.017

中图分类号：

学科分类号：

摘要：

710 samples of tobacco plants showing mosaic symptom were collected from different tobacco-growing areas in Yunnan province. 546 of the 710 samples were detected TMV antigen positive by TAS-ELISA. According to geographical distribution, 38 isolates were amplified, cloned and sequenced by IC-RT-PCR for TMV coat protein gene. Result showed that nucleotide sequences for coat protein of these 38 isolates were 95% to 100% similar while amino acid sequences were similar 97.5%~100%. The similarity among different TMV isolates in Yunnan and TMV-U1, TMV-Fujian were relatively high in nucleotide sequences of coat protein gene. The phylogenetic tree of TMV isolates showed TMV isolates in Yunnan share certain evolutionary relationship with TMV-U1 strain.

引用

页码：106 / 113

页数：7