In this study, the OptoTrak system was employed to collect the articulating surface measurements of the human knee for the femur, tibia and patella in three experimented specimens. Furthermore. a rigorous mathematical reconstruction procedure that estimates reconstruction error was completed by employed the relative analysis tools. The results show, the measurements for each session were able to reconstruct the three-dimensional calibration to a precision of 0.02mm. On the other word. the OptoTrak can be used to obtain the precise measurements of analytical surface of the human knee joint.