Enhanced production of spinosad with strains of Saccharopolyspora spinosa by genome shuffling

被引：0

作者：

Xia, Yanchun ^{[1
,2
]}

Wang, Chao ^{[2
]}

Chen, Yuan ^{[2
]}

Wu, Jianglei ^{[3
]}

Zou, Qiulong ^{[2
]}

Zhang, Xiaolin ^{[2
]}

Li, Chun ^{[1
]}

机构：

[1] Key Laboratory for Green Process of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, 832003, Xinjiang

[2] Academy of State Administration of Grain, Beijing

[3] School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, Hubei

来源：

Li, Chun | 1600年 / Materials China卷 / 65期

关键词：

Genome shuffling; Protoplast fusion; Saccharopolyspora spinosa; Spinosad;

D O I：

10.3969/j.issn.0438-1157.2014.09.036

中图分类号：

学科分类号：

摘要：

In this study, genome shuffling of eight original strains obtained by various methods of chemical and physical mutations was used to achieve rapid improvement of spinosad production. The conditions of preparation, regeneration, parents inactivation and fusion of protoplast of the original strain were investigated. The strain grown for 65 h was treated by 4 mg·ml-1 lysozyme at 39℃ for 20 min, and preparation rate and regeneration rate were 92.30% and 7.66%, respectively. The protoplasts were inactivated by heating at 60℃ for more than 90 min and UV treatment more than 200 s, respectively. The inactivated protoplasts were fused and regenerated in polyethylene glycol (PEG 6000, 50%) for 15 min, and fusion rate was about 1.18%. After three rounds of genome shuffling, a high yielding strain, designated as S. spinosa 3-652, was isolated. The increased yield was about 36.07% higher than that of the original strain. Subculture experiments indicated that the high producers were stable. © All Rights Reserved

引用

页码：3576 / 3582

页数：6

共 20 条

[1] Jin Z.H., Xu B., Lin S.Z., Jin Q.C., Cen P.L., Enhanced production of spinosad in Saccharopolyspora spinosa by genome shuffling , Applied Biochemistry and Biotechnology, 159, 3, pp. 655-663, (2009)
[2] Susan C.V., Pharm D., Robin L.B., Pharm D., Head lice and the use of spinosad , Clinical Therapeutics, 1, pp. 14-23, (2012)
[3] Luo L., Li N., Li L., Guo W., Yu J., Zhang X., Studies on high throughput screening for high spinosad-producing strains with 96-well plates , Journal of Agricultural Science and Technology, 12, 2, pp. 133-137, (2010)
[4] Chen Y., Xiong J., Wang C., Eddie C., Zou Q., Zhang X., Rapid screening method of high yield strains spinosad , Science and Technology of Cereals, Oils and Foods, 21, 4, pp. 99-102, (2013)
[5] Sharma A., Strivastava A., Ram B., Srivastava P.C., Dissipation behavior of spinosad insecticide in soil, cabbage and cauliflower under subtropical conditions , Pest Management Science, 7, 12, pp. 2611-2617, (2008)
[6] Chen Y., Xiong J., Guo W., Liu G., Zou Q., Zhang X., High throughput screening and breeding of high spinosad producing strains , Chinese Journal of Antibiotics, 38, 5, pp. 339-347, (2013)
[7] Vayias B.J., Athanassiou C.G., Milonas D.N., Mavrotasb C., Activity of spinosad against three stored-product beelte species on four grain commodities , Crop Protection, 28, 7, pp. 561-566, (2009)
[8] Gao X.F., Zhao H., Zhang G.H., He K.Z., Jin Y.L., Genome shuffling of clostridium acetobutylicum CICC 8012 for improved production of acetone-butanol-ethanol (ABE) , Current Microbiology, 65, 2, pp. 128-132, (2012)
[9] Zhang Y.X., Perry K., Vinci V.A., Powell K., Stemmer W.P., del Cardayre S.B., Genome shuffling leads to rapid phenotypic improvement in bacteria , Nature, 415, 6872, pp. 644-646, (2002)
[10] Long M., Li P., Zhang W.K., Yi Z., Guo Y., Yang S.H., Wang Z., Liu G.W., Genome shuffling of Megasphaera elsdenii for improving acid-tolerance and propionate production , African Journal of Microbiology Research, 6, 18, pp. 4041-4047, (2012)

← 1 2 →