Clay-based affinity probes for selective cleanup and determination of aflatoxin B1 using nanostructured montmorillonite on quartz

被引：0
作者：
Huebner, Henry J. [1 ]
Phillips, Timothy D. [1 ]
机构：
[1] Department of Veterinary Anatomy, Faculty of Toxicology, Texas A and M University, College Station
来源：
| 2003年 / AOAC International卷 / 86期
关键词：
Adsorbents - Decontamination - Enthalpy - Fluorescence - Liquid chromatography - Nanostructured materials - Quartz;
D O I：
10.1093/jaoac/86.3.534
中图分类号：
学科分类号：
摘要：
A study was conducted to investigate the selective cleanup and determination of aflatoxin B1 (AfB1) from contaminated media. Composite adsorbents were formulated from calcium montmorillonite clay, which possesses a high affinity and enthalpy of adsorption for AfB1. Nanostructuring techniques were used to construct various formulations of the clay-based composite media. In AfB1 adsorption studies with prototypical affinity columns, these composites offered narrowly defined, reproducible capacity ranges. Composite recoveries of AfB1 from spiked grains exhibited linear trends that correlated well with the range of spike levels. Composite columns provided lower recoveries of AfB1 from naturally contaminated corn than did immunoaffinity columns; however, recoveries were consistent and purified extracts were free of interfering compounds, as determined by liquid chromatography with fluorescence detection.
引用
收藏
页码：534 / 539
页数：5
相关论文
共 25 条
  • [1] IARC Monographs on the Evaluation of Carcinogenic Risks of Chemicals to Humans: Some Naturally Occurring Substances: Food Items and Constituents, Heterocyclic Aromatic Amines and Mycotoxins, 56, pp. 245-395, (1993)
  • [2] Candlish A.A.G., Stimson W.H., Chromatography of Mycotoxins: Techniques and Applications, pp. 99-123, (1993)
  • [3] Jaimez J., Fente C.A., Vazquez B.I., Franco C.M., Cepeda A., Mahuzier G., Prognon P., J. Chromatogr. A, 882, pp. 1-10, (2000)
  • [4] Scott P.M., Trucksess M.W., J. AOAC Int., 80, pp. 941-949, (1997)
  • [5] Lvov Y., Haas H., Decher G., Mohwald H., Mikhailov A., Mtchedlishvily B., Morgunova E., Vainshtein B., Langmuir, 10, pp. 4232-4236, (1994)
  • [6] Dekany I., Turi L., Tombacz E., Pendler J.H., Langmuir, 11, pp. 2285-2292, (1995)
  • [7] Freeman R.G., Grabar K.C., Allison K.J., Bright R.M., Davis J.A., Guthrie A.P., Hommer M.B., Jackson M.A., Smith P.C., Walter D.G., Natan M.J., Science, 267, pp. 1629-1632, (1995)
  • [8] Grabar K.C., Freeman R.G., Hommer M.B., Natan M.J., Anal. Chem., 67, pp. 735-743, (1995)
  • [9] Kleinfeld E.R., Ferguson G.S., Chem. Mater., 7, pp. 2327-2331, (1995)
  • [10] Kotov N.A., Dekany I., Fendler J.H., J. Phys. Chem., 99, pp. 13065-13069, (1995)
123