Distribution of the lipopeptide biosurfactant-producing microbes in oil reservoir environment

被引：0

作者：

Key Laboratory of Marine Chemistry Theory and Technology, College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao ^{[1
]}

Shandong

266100, China

不详 ^{[2
]}

Shandong

257000, China

不详 ^{[3
]}

100728, China

机构：

[1] Key Laboratory of Marine Chemistry Theory and Technology, College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao, 266100, Shandong

[2] Oil Production Research Institution, Sinopec Shengli Oilfield Company, Dongying, 257000, Shandong

[3] China Petroleum & Chemical Corporation, Beijing

来源：

Shiyou Xuebao | / 9卷 / 1122-1126期

关键词：

Biosurfactant; Enhancy oil recovery; Gene; Lipopeptide; Oil reservoir;

D O I：

10.7623/syxb201509010

中图分类号：

学科分类号：

摘要：

Through tests on the synthetase gene (srfA/licA) of lipopeptide surfactants such as surfactin and bacillus licheniformis, the distribution of lipopeptide surfactant-producing microbes in oil reservoirs of Shengli oilfield at 55-95℃ was analyzed in terms of function gene level. Meanwhile, clone library analysis was carried out on oil reservoir samples. The results indicated that there were a variety of different types of bacteria in the reservoir samples with high diversity. Among the produced fluid samples in total eight oil wells of four oil reservoirs, srfA/licA was detected out in six oil wells of three oil reservoirs. The content of srfA/licA was increased for all samples after oscillation enrichment culture. The microbes producing lipopeptide surfactant were prevalent in oil reservoirs of Shengli oilfield under different temperatures. It is speculated that the bacterial species producing lipopeptide surfactant include Bacillus, Pseudomonas, Serratia, Acinetobacter and Arcobacter. Therefore, the injection of microbes producing lipopeptide surfactant in oil reservoirs is an effective way to enhance oil recovery using biosurfactant. ©, 2015, Science Press. All right reserved.

引用

页码：1122 / 1126

页数：4

共 22 条

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