Studies on Mutagenesis Bacillus cereus Producing Phospholipase C

被引：0
|
作者：
Song Y. [1 ]
Wang T. [2 ]
Li D. [2 ]
Yan S. [2 ]
Yu D. [2 ]
Zhang K. [3 ]
Wang J. [1 ]
机构：
[1] Jinlin Business and Technology College, Changchun
[2] College of Food Science and Technology, Northeast Agricultural University, Harbin
[3] Henan Academy of Agricultural Sciences, Institute of Agricultural Products Processing, Zhengzhou
来源：
Zhang, Kangyi (kangyiz@163.com) | 2018年 / Chinese Institute of Food Science and Technology卷 / 18期
关键词：
Bacillus cereus; Enzyme activity; Mutation; Phospholipas C;
D O I：
10.16429/j.1009-7848.2018.03.019
中图分类号：
学科分类号：
摘要：
The departure strains of Bacillus cereus are handling by three kinds of mutagenesis, and get a phospholipase C high-yield strains of B.cereus-3. Selection of the precipitation ring diameter and the number of viable counts as a reference. By response surface to optimize the fermentation conditions of the strain B. cereus-3, the result for that: the inoculum size is 2.5%, speed is 160 r/min, fermentation temperature is 30.5℃ and the fermentation time is 18.5 h. At the time, the precipitation ring diameter is maximum, and phospholipase C activity is 26.3 U/mL. The crude enzyme solution were isolated and purified, and used SDS-PAGE method to measure the molecular weight of the purified phospholipase C, molecular weight between about 29-34 ku. © 2018, Editorial Office of Journal of CIFST. All right reserved.
引用
收藏
页码：141 / 149
页数：8
相关论文
共 10 条
  • [1] Aloulou A., Ali Y.B., Bezzine S., Et al., Phospholipases: an overview, Methods in Molecular Biology, 861, 861, pp. 63-85, (2012)
  • [2] Rhee S.G., Regulation of phosphoinositide-specific phospholipase C, Annu Rev Biochem, 70, pp. 281-312, (2001)
  • [3] Fukami K., Inanobe S., Kanemaru K., Et al., Phospholipase C is a key enzyme regulating intracellular calcium and modulating the phosphoinositide balance, Progress in Lipid Research, 49, 4, pp. 429-437, (2010)
  • [4] Jiang X.F., Chang M., Jin Q., Et al., Application of phospholipase A1 and phospholipase C in the degumming process of different kinds of crude oils, Process Biochemistry, 50, 3, pp. 432-437, (2015)
  • [5] Buchold H., Boensch R., Schroeppel J., Process for enzymatically degumming vegetable oil
  • [6] Jiang X.F., Chang M., Wang X.S., Et al., Effect of ultrasound treatment on oil recovery from soybean gum by using phospholipase C, Journal of Cleaner Production, 69, pp. 237-242, (2014)
  • [7] Ikezawa H., Matsushita M., Tomita M., Et al., Effeets of metalions on sphingomyelinase activity of Bacillus cereus, Arch Bioehem BioPhys, 249, pp. 588-595, (1986)
  • [8] Titball R.W., Hunter S.E.C., Mart N.K.L., Et al., Molecular cloning and nucleotide sequence of the alpha-toxin(phospholipaseC) of Clostridium perfiingens, Infect Immun, 57, 2, pp. 367-376, (1989)
  • [9] Jiang X., Chang M., Wang X., Et al., Effect of ultrasound treatment on oil recovery from soybean gum by using phospholipase C, Journal of Cleaner Production, 69, pp. 237-242, (2014)
  • [10] Zheng H., Zhang C., Qin X., Et al., Study on the protein fractions extracted from the muscle tissue of Pinctada martensii and their hydrolysis by pancreatin, International Journal of Food Science & Technology, 47, 10, pp. 2228-2234, (2012)
1