Structural Characterization, Antioxidant and Antibacterial Activities of Polysaccharides from Blueberry

被引：1

作者：

Qiao, Yanyan ^{[1
]}

Jiang, Hongzhou ^{[2
]}

Li, Dongnan ^{[1
]}

Li, Bin ^{[1
]}

机构：

[1] Food Science College, Shenyang Agricultural University, Shenyang

[2] Anhui Ziyue Biotechnology Co. Ltd., Wuhu

来源：

Shipin Kexue/Food Science | 2024年 / 45卷 / 21期

关键词：

antibacterial activity; antioxidant activity; blueberry; polysaccharide; structural characterization;

D O I：

10.7506/spkx1002-6630-20231209-079

中图分类号：

学科分类号：

摘要：

A neutral polysaccharide (BPN1) was obtained from blueberry by citric acid-assisted extraction followed by purification through cellulose DEAE-52 and Sephadex G-100 column chromatography. The average weight molecular mass (mw) of BPN1, as determined by high-performance size exclusion chromatography with multi-angle laser light scattering (HPSEC-MALLS) detection, was 2.991 × 104 Da, the polydispersity index (PDI) was 1.163, and the molecular mass distribution ranged narrowly. Monosaccharide composition analysis performed using 1-phenyl-3-methyl-5-pyrazolone derivatization followed by high performance liquid chromatography (HPLC) showed that BPN1 was mainly composed of glucose, galactose, xylose and araban. The glycosidic bond configuration and linkage of BPN1 were analyzed by infrared spectroscopy and gas chromatography-mass spectrometry (GC-MS) after methylation. Combining the obtained results with two-dimensional nuclear magnetic spectroscopy (NMR) analysis, it was inferred that the polysaccharide was composed of a backbone chain of 1→4 linked dextran, some of which were substituted at position C6 by araban, xylose and galactooligosaccharide. The in vitro antioxidant assays showed that BPN1 had stronger scavenging capacity against 1,1-diphenyl-2-picrylhydrazyl, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation and hydroxyl radicals than hot water-soluble blueberry polysaccharide. The antibacterial test showed that the minimum inhibitory concentration (MIC) of BPN1 against Staphylococcus aureus and Escherichia coli was in the range of 2.5 to 5 mg/mL, and it exerted antibacterial activity against S. aureus and E. coli by destroying the cell membrane. This study provides a basis for the structural analysis, development and utilization of blueberry polysaccharides. © 2024 Chinese Chamber of Commerce. All rights reserved.

引用

页码：94 / 103

页数：9

共 30 条

[1]

SIVAPRAGASAM N, NEELAKANDAN N, VASANTHA RUPASINGHE H P., Potential health benefits of fermented blueberry: a review of current scientific evidence, Trends in Food Science & Technology, 132, pp. 103-120, (2023)

[2]

SILVA S, COSTA E M, VEIGA M, Et al., Health promoting properties of blueberries: a review, Critical Reviews in Food Science and Nutrition, 60, 2, pp. 181-200, (2020)

[3]

HOTCHKISS A T, CHAU H K, STRAHAN G D, Et al., Structure and composition of blueberry fiber pectin and xyloglucan that bind anthocyanins during fruit puree processing, Food Hydrocolloids, 116, (2021)

[4]

31, 17, (2010)

[5]

FENG L Y, ZHOU Y, ASHAOLU T J, Et al., Physicochemical and rheological characterization of pectin-rich fraction from blueberry (Vaccinium ashei) wine pomace, International Journal of Biological Macromolecules, 128, pp. 629-637, (2019)

[6]

31, 22, (2010)

[7]

YUAN Q X, ZHANG J, XIAO C L, Et al., Structural characterization of a low-molecular-weight polysaccharide from Angelica pubescens Maxim. f. biserrata Shan et Yuan root and evaluation of its antioxidant activity, Carbohydrate Polymers, 236, (2020)

[8]

JIANG L, WANG W J, WEN P W, Et al., Two water-soluble polysaccharides from mung bean skin: physicochemical characterization, antioxidant and antibacterial activities, Food Hydrocolloids, 100, (2020)

[9]

LI W, YUN L Y, RIFKY M, Et al., Carboxymethylation of (1→ 6)-α-dextran from Leuconostoc spp.: effects on microstructural, thermal and antioxidant properties, International Journal of Biological Macromolecules, 166, pp. 1-8, (2021)

[10]

LV Q Q, CAO J J, LIU R, Et al., Structural characterization, α-amylase and α-glucosidase inhibitory activities of polysaccharides from wheat bran, Food Chemistry, 341, (2021)

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