Silver ion solid-phase extraction chromatography for the analysis of trans fatty acids

被引：17

作者：

Kraft, Jana ^{[1
]}

Kramer, John K.G. ^{[1
]}

Hernandez, Marta ^{[1
]}

Letarte, Jocelyne ^{[1
]}

Aldai, Noelia ^{[1
]}

Sandercole, Viviana ^{[1
]}

Mohammed, Riazuddin ^{[1
]}

Mayer, Franz ^{[1
]}

Mossoba, Magdi M. ^{[1
]}

Delmonte, Pierluigi ^{[1
]}

机构：

[1] Department of Animal Science, University of Vermont, Burlington, VT

来源：

Lipid Technology | 2014年 / 26卷 / 02期

关键词：

Column chromatography - Isomers - Phase separation - Silicones - Silver - Extraction - Metal ions - Fatty acids;

D O I：

10.1002/lite.201400008

中图分类号：

学科分类号：

摘要：

This article describes the application of silver ion solid-phase extraction (Ag+-SPE) to the separation of the fatty acid methyl esters (FAMEs) prepared from a partially hydrogenated fish oil (PHFO) with a trans fatty acid (FA) content of 41%. The complex FAME mixture was resolved into the following FAME fractions: saturated FA, trans-monounsaturated FA (trans-MUFA), cis-MUFA, and several polyunsaturated FA (PUFA) fractions with mixed geometric and positional isomers. Fractions were analyzed by gas chromatography (GC) using a 100 m cyanopropyl polysiloxane (CPS) coated capillary column. The GC analysis of the Ag+-SPE fractions allowed for the identification of the transand cis-MUFA and other FAME prepared from PHFO. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：39 / 42

页数：3

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