GC-MS Analysis of Chemical Composition of in vivo Plant, in vitro and Elicited Roots of Bacopa monnieri (L.) Pennell

被引：0

作者：

Plant Tissue Culture and Bioprospecting Laboratory, M.S. Swaminathan Research Foundation, 3rd, Cross Road, Institutional Area, Taramani, Chennai ^{[1
]}

600 113, India

机构：

[1] Plant Tissue Culture and Bioprospecting Laboratory, M.S. Swaminathan Research Foundation, 3rd, Cross Road, Institutional Area, Taramani, Chennai

来源：

Eganathan, Palanisami (eganathan@gmail.com) | 1600年 / Taylor and Francis Ltd.卷 / 03期

关键词：

Bacopa monnieri; elicitation; hairy root; higher alkens; in vitro; in vivo; sesquiterpenoids;

D O I：

10.1080/22297928.2013.797636

中图分类号：

学科分类号：

摘要：

In vitro plant was developed by tissue culture utilizing nodal explants and adventitious roots were obtained from nodal explants of Bacopa monnieri in Murashige and Skoog (MS) medium supplemented with 0.2 mg/l-1 NAA. In vitro roots of Bacopa monnieri were subjected to chemical elicitation using 69 mg/l-1 of salicylic acid by hairy root culture. In vivo whole plant, in vitro and elicited roots were subjected to solvent extraction using ethanol and their chemical compounds analyzed using gas chromatography and mass spectroscopy (GC-MS). The major composition of in vivo plant were 9,12-octadecadienonic acid (36.96 %), 9,17-octadecadienal (26.65 %,), 9-octadecenoic acid (7.79 %) and in vitro roots yielded 9,12-octadecadienonic acid (25.62 %), 9-octadecenoic acid (23 %), 9,17-octadecadienal (16.08 %). In vitro roots subjected to salicylic elicitation comprised of 1,3-dihydroxyacetone dimmer (15.69 %), 1-hexadecena (7.74 %), 1-tetradecene (6.78 %), eicosane (6.57%), 1-octadecene (5.29 %), 1-decene (4.60 %), E-15-heptadecenal (4.45 %) and heptacosane (3.45 %). In vitro elicited roots showed thirty six compounds and increased higher percentage of sesquiterpenoids and higher alkens. © 2013 Har Krishan Bhalla & Sons.

引用

页码：380 / 388

页数：8

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