Evaluation of Sinensetin Derived From Flavonoids-Rich Orthosiphon Stamineus as a New Radiosensitizer in MDA-MB-231 and T47D as Breast Cancer Cell Lines

被引：0
作者：
Rezakhani N. [1 ]
Goliaei B. [2 ]
Mahdizade F. [3 ]
Nikoofar A. [4 ]
Neghab H.K. [5 ]
Soheilifar M.H. [6 ]
Azadeh S.S. [5 ]
Bigdeli B. [2 ]
机构：
[1] Department of Biology, Science and Research Branch, Islamic Azad University, Tehran
[2] Department of Biophysics, Institute of Biochemistry and Biophysics, University of Tehran, Tehran
[3] Department of Biology, Islamshahr Branch, Islamic Azad University, Tehran
[4] Department of Radiotherapy, Iran University of Medical Sciences (IUMS), Tehran
[5] Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute, ACECR, Tehran
[6] Department of Medical Laser, Medical Laser Research Center, Yara Institute, ACECR, Tehran
来源：
Iranian Journal of Medical Physics | 2023年 / 20卷 / 02期
关键词：
Breast Cancer; Flavonoids; Radio Sensitizer Agent; Sinensetin; X-Radiation;
D O I：
10.22038/IJMP.2022.64397.2104
中图分类号：
学科分类号：
摘要：
Introduction: Breast cancer is one of the most prevalent diseases around the world. Breast cancer patients treated with radiation may face Side effects as well as cancer recurrence. Some polyphenols exhibit antioxidant effects. Citrus and Orthosiphon Stamineus have source of methylated flavone called Sinensetin (SIN). In this research, we designation of Sinensetin as increasing radiation sensitivity. Material and Methods: The cytotoxic effect of Sinensetin was examined in MDA-MB-231 and T47D by MTT assay. As well as, the clonogenic ability of cells were Assessmented in the presence of Sinensetin and combination with radiation. To quantify expression alterations of apoptosis related genes, utilized Real-Time PCR method. Results: In a dose and time-dependent manner, Sinensetin decreased the viability of MDA-MB-231 and T47D. The survival fraction was decreased in cells treated with Sinensetin (SIN) prior to X-irradiation compared to cells treated with X-ray only. More ever, expression level of, Bcl-2, STAT3, and increased P53 via treated cells with Sinensetin (SIN) and X-ray. Conclusion: Due to the results, Sinensetin (SIN) can be mentioned as a novel radiosensitizer and its effects may considered increasing apoptosis following DNA damage induced by irradiation. © 2023.
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页码：114 / 119
页数：5
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