Expression of Cellular FLICE-like Inhibitory Protein in Granulosa Cells in In Vitro Fertilization Patients with Advanced Endometriosis

被引:0
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作者
Zhang Jian-Ping
Yan Mei-Qin
Wang Yao-Qin
Yang Fang
Liu Juan
Wu Xue-Qing
机构
[1] HumanAssistedReproductionCenter,ShanxiWomenandChildren’sHospital,Taiyuan,China
关键词
Antral Follicle Count; Cellular FLICE-like Inhibitory Protein; Clinical Pregnancy; Endometriosis; In Vitro Fertilization-Embryo Transfer;
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中图分类号
R714.8 [];
学科分类号
摘要
Background: This study evaluated the expression of cellular FLICE-like inhibitory protein (cFLIP) in granulosa cells (GCs) obtained fromin vitro fertilization-embryo transfer patients with advanced endometriosis.Methods: A total of 267 patients with advanced endometriosis were enrolled in this study. They were divided into clinical pregnancy group (n = 114) and nonpregnancy group (n = 153). The expressions of cFLIP in mRNA and protein level were measured by real-time polymerase chain reaction (RT-PCR) and Western blotting. The related factors on the clinical pregnancy were analyzed using logistic regression analysis. Coefficients of correlation were calculated using the nonparametric rho-Spearman test.Results: The number of oocytes retrieved, fertilization rate, and cleavage rate were significantly and independently related with clinical pregnancy (P > 0.05). RT-PCR and Western blotting analysis showed that the expressions of cFLP in mRNA and protein level were significantly higher in the clinical pregnancy group than in nonpregnancy group (P > 0.05). cFLIP had a significantly positive correlation with the number of oocytes retrieved (P > 0.05) and no correlation with fertilization rate and cleavage rate (P < 0.05).Conclusion: Higher expression of cFLIP increased the pregnancy rate in women with advanced endometriosis.
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