Purification, Identification, and Characterization of Surfactin from Bacillus velezensis 1-3

被引：3

作者：

Zhang J. ^{[1
,2
]}

Zhang L. ^{[1
]}

Tang W. ^{[1
,3
]}

Sun X. ^{[1
,2
]}

Tang T. ^{[1
,3
]}

Qiao X. ^{[1
,2
]}

Liang M. ^{[4
]}

He Z. ^{[2
,3
]}

机构：

[1] School of Medicine and Pharmacy, Ocean University of China, Qingdao

[2] Qingdao Bioantai Biotechnology Co. Ltd., Qingdao

[3] Qingdao Marine Biomedical Research Institute Co. Ltd., Qingdao

[4] Shandong Center for Quality Control of Feed and Veterinary Drugs, Jinan

来源：

Shipin Kexue/Food Science | 2023年 / 44卷 / 04期

关键词：

antimicrobial activity; Bacillus velezensis; cyclic lipopeptide; surfactin;

D O I：

10.7506/spkx1002-6630-20220326-326

中图分类号：

学科分类号：

摘要：

The antimicrobial substances produced by Bacillus velezensis 1-3, a strain with antibacterial activity isolated from oral samples, were separated and purified by ammonium sulfate precipitation, gel filtration chromatography and thin-layer chromatography and were analyzed by matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS). These substances were identified as surfactin consisting of three cyclic lipopeptides with 14–16 carbon atoms, whose molecular masses were 1 030.642, 1 044.660 and 1 058.677 Da, respectively. Surfactin could function as a surfactant and exhibited good antimicrobial activity against common pathogenic bacteria in the food, medical and livestock industries. Meanwhile, the minimal inhibitory concentration (MIC) of surfactin against Listeria monocytogenes ATCC 19115 was 4 μg/mL. Surfactin did not show hemolytic activity against mouse red blood cells within the effective antibacterial concentration range. It could endure temperatures at 20–100 ℃ and withstand a wide pH range of 2.0–10.0. Hence, surfactin produced by B. velezensis 1-3 has great potential for applications in the food, medical, and livestock fields. © 2023 Chinese Chamber of Commerce. All rights reserved.

引用

页码：177 / 184

页数：7

共 24 条

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