Optimization of Ultrasonic-Assisted Extraction of Polysaccharides from Polyporus umbellatus Sclerotium by Response Surface Methodology and Antioxidant Activity

被引：0

作者：

Zheng Y. ^{[1
,2
]}

Zhang A. ^{[3
]}

Zhang X. ^{[2
,4
]}

Mei G. ^{[2
,4
]}

He P. ^{[2
,4
]}

机构：

[1] College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan

[2] Zhejiang Marine Fisheries Research Institute, Zhoushan

[3] College of Food Science and Technology, Zhejiang University of Technology, Hangzhou

[4] Zhejiang Province Key Lab of Mariculture & Enhancement, Zhoushan

来源：

Science and Technology of Food Industry | 2023年 / 44卷 / 16期

关键词：

antioxidant activity; DPPH free radical; optimization of extraction process; Polyporus umbellatus sclerotia; polysaccharide; reduction power;

D O I：

10.13386/j.issn1002-0306.2022110343

中图分类号：

学科分类号：

摘要：

To promote extraction efficiency of bioactive polysaccharides from sclerotium of Polyporus umbellatus, ultrasonic-assisted extraction was applied to extract polysaccharides from P. umbellatus sclerotium. The effects of extraction time, ultrasonic power, extraction temperature and liquid-to-material ratio on the yield of polysaccharide were investigated by combining single factor experiments and response surface optimization, and the optimal extraction parameters were also determined. Meanwhile, antioxidant activities were evaluated by DPPH free radical scavenging ability and reducing power test. The results showed that the yield of polysaccharides from sclerotia of P. umbellatus was significantly correlated to the interaction between extraction temperature and ultrasonic power, extraction temperature and liquid-to-material ratio, ultrasonic power and liquid-to-material ratio, liquid-to-material ratio and extraction time. The optimum extraction parameters were extraction temperature 72.0 ℃, extraction power 300 W, extraction time 65 min and liquid-solid ratio 22 mL/g, and the maximum yield was 2.47%±0.03%. PUPF30, PUPF60 and PUPF80 were obtained from polysaccharide extracts by graded ethanol precipitation. Their polysaccharide contents were respectively 39.99%, 58.60% and 50.35%, and the protein contents were respectively 25.80%, 14.42% and 25.54%. Within the tested dose range, PUPF30, PUPF60 and PUPF80 showed the highest DPPH scavenging rate of 50.63%, 83.95%, 87.48%, respectively, and the highest reduction power of 0.828, 1.433, 1.525, respectively. The ultrasonic-assisted extraction optimized by response surface methodology was preferable for effective extraction and preparation of bioactive polysaccharides from sclerotia of P. umbellatu. This study would lay a foundation for the further development and utilization of polysaccharides from sclerotia of P. umbellatus. © 2023 Editorial Department of Science and Technology of Food Science. All rights reserved.

引用

页码：255 / 263

页数：8

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