Bio-electrochemical-system-assisted microbial reductive dechlorination of pentachlorophenol: Spectro-electrochemical analyses of cathode biofilm

被引：0
|
作者：
Tang J. [1 ]
Wen P. [1 ,2 ]
Liu M. [3 ]
Yu Z. [1 ]
Cai X. [1 ,2 ]
Tang J. [1 ]
机构：
[1] Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Science & Technology, Guangzhou
[2] Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou
[3] Key Laboratory of Renewable Energy and Gas Hydrate, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou
来源：
Zhongguo Kexue Jishu Kexue/Scientia Sinica Technologica | 2019年 / 49卷 / 12期
关键词：
Cathode biofilm; Electron transfer; In situ electrochemical FTIR; Pentachlorophenol; Reductive dechlorination;
D O I：
10.1360/SST-2019-0064
中图分类号：
学科分类号：
摘要：
Microbial reductive dechlorination of pentachlorophenol (PCP) was facilitated by a bio-electrochemical system (BES) with the electrode as the direct electron donor under various constant potentials (0, -200, -500, and -800 mV vs. standard hydrogen electrode (SHE)). We detected the dechlorination efficiency, the electron acceptability, and the electroactive substances of the cathode biofilms. The results showed that the lower potentials promoted PCP dechlorination owing to the greater electron uptake rates in the BES with lower potentials and that cytochrome OmcZ could be the main electroactive mediator to participate in the PCP dechlorination in BES at the potential of -500 mV. In situ electrochemical FTIR spectra and two-dimensional correlation spectroscopy analysis (2DCOS) were employed to obtain a comprehensive understanding of the redox mediators and the related response to electrons on the cathode biofilms. The results demonstrated that amide III, c-type cytochromes, and humic acids might facilitate electron transfer from the electrode to dechlorinators under potentials of -200, -500, and -800 mV, respectively. Compared with amide III and humic acids, c-type cytochromes could mediate more efficient electron transfer, resulting in better performance of the microbial reductive dechlorination of PCP. Additionally, the microbial community structure from 16S rRNA gene sequencing revealed that the lower potentials significantly enriched Comamonadaceae with an abundance of more than 70%, which could be the main driving force for electron transfer in the reductive dechlorination of PCP on the cathode biofilms. © 2019, Science Press. All right reserved.
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页码：1483 / 1494
页数：11
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