Analysis of Quality Characteristics of Guizhou Black Tea Based on UPLC-Q-Orbitrap High-resolution Mass Spectrometry

被引:0
作者
Jiang X. [1 ,2 ,3 ]
Tao H. [1 ]
Song J. [1 ]
Xu C. [1 ]
Sun X. [1 ]
Li Z. [2 ]
Yang H. [1 ]
机构
[1] School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang
[2] Guizhou Academy of Testing and Analysis, Guiyang
[3] Department of Vector Disinfection, Kunming City Center for Disease Control and Prevention, Kunming
关键词
differential metabolites; Guizhou black tea; high-resolution mass spectrometry; principal component analysis;
D O I
10.16429/j.1009-7848.2023.05.034
中图分类号
学科分类号
摘要
Guizhou black tea has excellent quality due to its ecological advantages. In order to have an insight into the quality characteristics of Guizhou black tea, non-targeted metabonomics analysis for the nonvolatile tea metabolite of three representative of Guizhou black tea (Zunyi red tea̖ Púan red tea̖ Shiqian moss tea)was conducted by ultra-performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry (UPLC-Q-Orbitrap). The results showed that 96 metabolites contained in three kinds of Guizhou black teas were identified through chromatographic mass spectrometry analysis, including flavonoids, amino acids and their derivatives, nucleotides and their derivatives, and phenolic acids. According to the principal component analysis (PCA), the three kinds of Guizhou black tea can be effectively distinguished, and it is concluded that there were significant differences between different varieties of black tea. Then, 23 kinds of differential metabolites among the three kinds of Guizhou black tea were screened out according to the FC value, P value and VIP value. Metabolic pathway analysis shows that its main metabolic pathway is the metabolism of flavonoids, which may be the reason for the differences in the taste of the three kinds of black teas. This study revealed the differential metabolites of the three kinds of Guizhou black teas from the perspective of metabolomics, and provided a reference for the identification of tea varieties. © 2023 Chinese Institute of Food Science and Technology. All rights reserved.
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页码:341 / 351
页数:10
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