Effects of substitution of ER domains on the synthesis of eicosapentaenoic acid in Schizochytrium limacinum SR21

被引：0
|
作者：
Yang R. [1 ]
Zheng X. [1 ]
Lu T. [1 ]
Zhao Y. [1 ]
Yang Q. [1 ]
Lu Y. [1 ,2 ,3 ]
He N. [1 ]
Ling X. [1 ,2 ,3 ]
机构：
[1] College of Chemistry and Chemical Engineering, Xiamen University, Xiamen
[2] Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen
[3] The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen
来源：
Huagong Xuebao/CIESC Journal | 2021年 / 72卷 / 07期
关键词：
Enoyl-reductase; EPA; Fermentation; Metabolism; Molecular biology; Schizochytrium limacinum; Shewanella;
D O I：
10.11949/0438-1157.20201818
中图分类号：
学科分类号：
摘要：
Eicosapentaenoic acid (EPA) is widely used in the fields of food, medical treatment, cosmetics and feed additives because of its physiological functions of regulating blood lipids, reducing fibrin, preventing cardiovascular diseases, anti-inflammatory and anti-allergic. At present, the supply of EPA cannot meet the market demand, due to the natural resources such as fish oil rich in polyunsaturated fatty acids (PUFAs) are gradually scarce. Therefore, more and more research focus on using the modified microbial strains to produce EPA. In this study, homologous recombination technology was used to knock the enoyl-reductase gene (sh-ER) of Shewanella sp.SCRC2738 into the ORFB-ER and ORFC-ER domains of Schizochytrium limacinum SR21, in order to regulate the preference for polyunsaturated fatty acid synthesis to increase the yield of EPA. The results showed that the EPA content of the B-sh-ER strain increased by 85.7% and the transcriptional level of polyketide synthases (PKS) pathway related genes were significantly up-regulated. The synthesis of PUFAs in C-sh-ER strains presented little change. In 5 L fed-batch fermentation, total lipids yield of the B-sh-ER strains increased by 28.7% reaching to 73.2 g/L; the EPA yield increased by 71.6% reaching to 732.4 mg/L. This study supplies a new strategy for promoting the high production of PUFAs and provides a novel perspective for regulating the synthesis of EPA in Schizochytrium sp. © 2021, Chemical Industry Press Co., Ltd. All right reserved.
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页码：3768 / 3779
页数：11
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