Effects of Three Commonly Used Herbicides on Bacterial Antibiotic Resistance

被引：0
|
作者：
Li X. [1 ]
Liao H.-P. [1 ]
Cui P. [1 ]
Bai Y.-D. [1 ]
Liu C. [1 ]
Wen C. [1 ]
Zhou S.-G. [1 ]
机构：
[1] College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou
来源：
Huanjing Kexue/Environmental Science | 2021年 / 42卷 / 05期
关键词：
Antibiotic resistance; Escherichia coli; Genetic mutation; Herbicides; Mutation frequency;
D O I：
10.13227/j.hjkx.202009249
中图分类号：
学科分类号：
摘要：
The spread of antibiotic resistance has become a serious threat to global public health. Recently, several studies have found that non-antibiotic chemicals can promote the generation and spread of bacterial antibiotic resistance. However, the effects of herbicides on the antibiotic resistance of bacteria remain unclear. In this study, Escherichia coli DH5α was used as the model strain to explore the effects of three commonly used herbicides (glyphosate, glufosinate, and dicamba) on the antibiotic resistance under soil environmental concentrations. The results showed that herbicide exposure affected the sensitivity of E. coli DH5α to antibiotics and significantly improved the resistance of E. coli DH5α to gentamicin (glyphosate>dicamba>glufosinate). After 30 d of herbicide exposure, the E. coli mutant strains enhanced the resistance to tetracycline, chloramphenicol, and aminoglycoside antibiotics, and the minimum inhibitory concentration of streptomycin was increased by 19.8 times. The whole-genome sequencing results illustrated that herbicides induced several previously well-characterized mutations associated with membrane proteins (ompF and papC), fimbriae proteins (yraH), and ribosomes (rpsL) related to antibiotic resistance. Together, the results showed that herbicides can enhance the antibiotic resistance of bacteria via inducing genetic mutations, thereby promoting the potential risk of the spread of antibiotic resistance genes in the environment. © 2021, Science Press. All right reserved.
引用
收藏
页码：2550 / 2557
页数：7
相关论文
共 42 条
  • [1] O'Neill J., Antimicrobial Resistance: Tackling a Crisis for the Health and Wealth of Nations, pp. 1-16, (2014)
  • [2] Liao J, Wei X Q, Xiao Y Q, Et al., Pollution characteristics and risk assessment of antibiotics in Lianhua Reservoir, Environmental Scinence, 41, 9, pp. 4081-4087, (2020)
  • [3] Qiao M, Ying G G, Singer A C, Et al., Review of antibiotic resistance in China and its environment, Environment International, 110, pp. 160-172, (2018)
  • [4] Peng Q, Wang W Z, Xu W H., Ecological risk assessment of tetracycline antibiotics in livestock manure and vegetable soil of Chongqing, Environmental Science, 41, 10, pp. 4757-4766, (2020)
  • [5] Galan J C, Gonzalez-Candelas F, Rolain J M, Et al., Antibiotics as selectors and accelerators of diversity in the mechanisms of resistance: from the resistome to genetic plasticity in the β-lactamases world, Frontiers in Microbiology, 4, (2013)
  • [6] Zhang J P, Li W Y, Chen J P, Et al., Impact of disinfectant on bacterial antibiotic resistance transfer between biofilm and tap water in a simulated distribution network, Environmental Pollution, 246, pp. 131-140, (2019)
  • [7] Zhang Y, Gu A Z, Cen T Y, Et al., Petrol and diesel exhaust particles accelerate the horizontal transfer of plasmid-mediated antimicrobial resistance genes, Environment International, 114, pp. 280-287, (2018)
  • [8] Li G Y, Chen X F, Yin H L, Et al., Natural sphalerite nanoparticles can accelerate horizontal transfer of plasmid-mediated antibiotic-resistance genes, Environment International, 136, (2020)
  • [9] Lu J, Wang Y, Jin M, Et al., Both silver ions and silver nanoparticles facilitate the horizontal transfer of plasmid-mediated antibiotic resistance genes, Water Research, 169, (2020)
  • [10] Wang Q, Mao D Q, Luo Y., Ionic liquid facilitates the conjugative transfer of antibiotic resistance genes mediated by plasmid RP4, Environmental Science & Technology, 49, 14, pp. 8731-8740, (2015)
12345