Cell factory construction accelerated by genome synthesis and rearrangement

被引：0
作者：
Xie Z. [1 ,2 ]
Chen X. [1 ,2 ]
Xiao W. [1 ,2 ]
Li B. [1 ,2 ]
Yuan Y. [1 ,2 ]
机构：
[1] Frontier Science Center for Synthetic Biology, Ministry of Education, Tianjin
[2] Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin
来源：
Huagong Xuebao/CIESC Journal | 2019年 / 70卷 / 10期
关键词：
Cell factory; Chassis; Genome rearrangement; Genome synthesis; Metabolic pathway;
D O I：
10.11949/0438-1157.20190702
中图分类号：
学科分类号：
摘要：
Cell factories can use microbial cells to produce the energy, drugs, and chemicals that humans need. The adaptation of chassis cells to exogenous metabolic pathways is a core challenge in building efficient cell factories. Genome synthesis refers to the bottom-up construction of a genome from chemically synthesized nucleotides. Genomic induced rearrangement refers to the customized regulation of DNA sequences and structures in genome scale. Genome synthesis and inducible rearrangement have enabled the creation of organisms, enhanced the flexibility of model chassis. Proper genome simplification and codon simplification improve the utilization efficiency of substrates and energy, and improve the predictability and controllability of cell physiological performance. Genomic rearrangement can generate structural variations such as random deletion, replication, translocation and inversion. The rearrangements of synthetic genome accelerate the rapid evolution of chassis and the optimization of metabolic pathways, and improve the adaptability between chassis and pathway. The genome synthesis and inducible rearrangement provide a new strategy for the construction and optimization of cell factories. © All Right Reserved.
引用
收藏
页码：3712 / 3721
页数：9
相关论文
共 70 条
  • [1] Breitling R., Takano E., Synthetic biology advances for pharmaceutical production, Curr. Opin. Biotechnol., 35, pp. 46-51, (2015)
  • [2] Chang M.C., Eachus R.A., Trieu W., Et al., Engineering Escherichia coli for production of functionalized terpenoids using plant P450s, Nat. Chem. Biol., 3, 5, pp. 274-277, (2007)
  • [3] Paddon C.J., Keasling J.D., Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development, Nat. Rev. Microbiol., 12, 5, pp. 355-367, (2014)
  • [4] Hong K.K., Nielsen J., Metabolic engineering of Saccharomyces cerevisiae: a key cell factory platform for future biorefineries, Cell Mol. Life Sci., 69, 16, pp. 2671-2690, (2012)
  • [5] Ostrov N., Landon M., Guell M., Et al., Design, synthesis, and testing toward a 57-codon genome, Science, 353, 6301, pp. 819-822, (2016)
  • [6] Fredens J., Wang K., De La Torre D., Et al., Total synthesis of Escherichia coli with a recoded genome, Nature, 569, 7757, pp. 514-518, (2019)
  • [7] Pennisi E., Building the ultimate yeast genome, Science, 343, 6178, pp. 1426-1429, (2014)
  • [8] Sliva A., Yang H., Boeke J.D., Et al., Freedom and responsibility in synthetic genomics: the synthetic yeast project, Genetics, 200, 4, pp. 1021-1028, (2015)
  • [9] Dymond J.S., Richardson S.M., Coombes C.E., Et al., Synthetic chromosome arms function in yeast and generate phenotypic diversity by design, Nature, 477, 7365, pp. 471-476, (2011)
  • [10] Annaluru N., Muller H., Mitchell L.A., Et al., Total synthesis of a functional designer eukaryotic chromosome, Science, 344, 6179, pp. 55-58, (2014)
1234567