Optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels

被引:17
|
作者
Ude M.U. [1 ]
Oluka I. [2 ]
Eze P.C. [2 ]
机构
[1] Engineering Research Development and Production, Projects Development Institute (PRODA), Emene, Enugu State
[2] Department of Agricultural and Bioresource Engineering, Enugu State University of Science and Technology (ESUT), Enugu State
来源
关键词
Cassava and potato peels; Enzymatic hydrolysis; Glucose; Optimization;
D O I
10.1016/j.jobab.2020.10.007
中图分类号
学科分类号
摘要
The optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels were carried out. The substrate was characterized using proximate analysis and hydrolysis. The process was optimized using response surface methodology while the kinetics of the hydrolysis was studied using Michealis-menten model. The results obtained showed that the mixed peels have high hemicellulose content, hydrolysable carbohydrate and cellulose. The optimum conditions for glucose yield of 79% by enzymatic hydrolysis are temperature of 36 °C, time of five days, pH of 4.55 and enzyme dosage of 0.428 g/50 mL. Enzymatic hydrolysis obeyed Michealis-Menten kinetic model with spontaneous and feasible reactions. Therefore, the results have proved that mixed peels (cassava and potato peels) is a good substrate for glucose production and study of other waste peels as feed-stock is recommended. © 2020 Published by Nanjing Forestry University.
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页码:283 / 290
页数:7
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